RECONSTRUCTED SKIN MODEL CONTAINING LANGERHANS CELLS
含有朗格汉斯细胞的重建皮肤模型
基本信息
- 批准号:2432859
- 负责人:
- 金额:$ 10万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-09-30 至 1998-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Many highly differentiated in vitro epidermal and dermal/epidermal skin
models have been developed, however, none have incorporated
immulnologically competent cells such as Langerhans cells (LC). An LC-
containing model would be used to study and predict allergic contract
dermatitis and hypersensitivity skin reactions. In addition, such a
model would find broad application in the immunology field since it is
now recognized that epidermal LC play a key role in host defense
mechanisms against skin cancer and bacterial, fungal, and viral
infections particularly human immunodeficiency virus (HIV).
Phase I research will investigate the development of a highly
differentiated epidermal model which contains LC, derived from neonatal
umbilical cord blood, and keratinocytes, derived from autologous
foreskins. LC precursor cells will be harvested, propagated, and
matured in vitro, and an effective means of incorporating LC in their
native (non-activated) state will be determined. The presence of LC
will be verified by immonohistochemistry and quantified using the
mixed lymphocyte reaction; ultrastructure will observed using
transmission electron microscopy. Finally, functional testing of the
model in which the effects of topical applied contact allergens and
irritants are compared will probe the utility of the model for studying
and predicting contact hypersensitivity.
PROPOSED COMMERCIAL APPLICATION: The proposed LC-containing
model would reduce or eventually replace the extended and costly
sensitization testing done on a myriad of consumer products, chemicals
and pharmaceuticals. In addition, it would provide researchers will be
valuable immunological tool in studying and developing therapies
against skin cancer and bacterial, fungal, and viral infections including
HIV.
许多高度分化的体外表皮和真皮/表皮皮肤
模型已经开发,但是,没有一个包含
免疫感受态细胞如朗格汉斯细胞(LC)。 信用证-
包含模型将用于研究和预测过敏性合同
皮炎和过敏性皮肤反应。 此外,这样一个
该模型将在免疫学领域得到广泛的应用,因为它是
现在认识到表皮LC在宿主防御中起关键作用
抗皮肤癌和细菌、真菌和病毒的机制
感染,特别是人类免疫缺陷病毒(HIV)。
第一阶段研究将调查一种高度
分化表皮模型,含有LC,来自新生儿
脐带血和角质形成细胞,来自自体
包皮 将收获LC前体细胞,增殖,并
在体外成熟,并将LC纳入其有效手段,
将确定本机(未激活)状态。 LC的存在
将通过免疫组织化学进行验证,并使用
混合淋巴细胞反应;超微结构观察
透射电子显微镜 最后,对
模型中,局部应用的接触性过敏原和
刺激物进行比较,以探讨该模型的实用性,
和预测接触性过敏。
拟议的商业应用:拟议的含LC
模型将减少或最终取代延长和昂贵的
对无数的消费品、化学品、
和药品。此外,它还将为研究人员提供
研究和开发治疗方法的重要免疫学工具
皮肤癌和细菌,真菌和病毒感染,包括
艾滋病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MITCHELL KLAUSNER其他文献
MITCHELL KLAUSNER的其他文献
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{{ truncateString('MITCHELL KLAUSNER', 18)}}的其他基金
Highly Differentiated Tissue Culture Models of Melanoma
黑色素瘤的高度分化组织培养模型
- 批准号:
6442624 - 财政年份:2002
- 资助金额:
$ 10万 - 项目类别:
In Vitro Skin Model for Percutaneous Absorption Studies
用于经皮吸收研究的体外皮肤模型
- 批准号:
6481054 - 财政年份:2002
- 资助金额:
$ 10万 - 项目类别:
HUMAN TISSUE MODEL FOR HETEROSEXUAL HIV INFECTION
异性 HIV 感染的人体组织模型
- 批准号:
6156165 - 财政年份:2000
- 资助金额:
$ 10万 - 项目类别:
Human Tissue Model For Heterosexual HIV Transmission
异性艾滋病毒传播的人体组织模型
- 批准号:
6486337 - 财政年份:2000
- 资助金额:
$ 10万 - 项目类别:
Human Tissue Model For Heterosexual HIV Transmission
异性艾滋病毒传播的人体组织模型
- 批准号:
6626084 - 财政年份:2000
- 资助金额:
$ 10万 - 项目类别:
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