Highly Differentiated Tissue Culture Models of Melanoma

描述（由申请人提供）：除肺癌外， 皮肤黑色素瘤（CM）的发病率增加速度比任何 其他癌症。目前的估计是，美国每75个人中就有一个人 在他或她的有生之年会感染CM。黑色素瘤的早期症状 然而，已经很好地描述了设计改进的治疗方法， 患者过上更长、更有成效的生活，更好地了解 CM的发展是必要的。这项提案的目的是为了提高 CM进展的各个阶段的分化的组织培养模型。 在I期期间，来自CM的各种疾病状态的细胞系将被培养。 在单层培养中扩增并表征。使用无血清培养 系统，这些细胞将被纳入一个器官型，全厚度 皮肤模型，以产生组织重建， 体内CM。组织将在形态学、肿瘤 进展和粘附受体。最后，组织的可重复性 将对模型进行研究。

DESCRIPTION (provided by applicant): With the exception of lung cancer in women, the incidence of cutaneous melanoma (CM) is increasing faster than any other cancer. Current estimates are that one of every 75 individuals in the US will contract CM during his or her lifetime. The various stages of melanoma have been well described, however, to devise improved treatments allowing patients to lead longer and more productive lives, a better understanding of progression of CM is needed. The purpose of this proposal is to produce highly differentiated, tissue culture models of the various stages of CM progression. During Phase I, cell lines from the various disease states of CM will be expanded in monolayer culture and characterized. Using a serum free culture system, these cells will be incorporated into an organotypic, full thickness skin model to produce tissue reconstructs which parallel the various stages of CM in vivo. The tissues will be characterized in terms of morphology, tumor progression, and adhesion receptors. Finally, reproducibility of the tissue models will be investigated.