Highly Differentiated Tissue Culture Models of Melanoma

资料说明(申请人提供)：除 在女性中，皮肤黑色素瘤(CM)的发病率比任何 其他癌症。目前的估计是，美国每75个人中就有一个 会在他或她的有生之年感染CM。黑色素瘤的不同阶段 然而，已经被很好地描述为设计出改进的治疗方法 让患者活得更长、更有成效，更好地了解 CM的进步是必要的。这项提议的目的是生产高质量的产品。 CM进展不同阶段的差异化组织培养模型。 在第一阶段，来自不同疾病状态的CM细胞株将被 在单层培养中扩增并鉴定。使用无血清培养 系统中，这些细胞将被结合到一个有机的、全厚度的 皮肤模型以产生组织重建，该组织重建平行于 Cm在体内。组织将在形态、肿瘤等方面进行表征 进展和黏附受体。最后，组织的重复性 将对模型进行调查。

DESCRIPTION (provided by applicant): With the exception of lung cancer in women, the incidence of cutaneous melanoma (CM) is increasing faster than any other cancer. Current estimates are that one of every 75 individuals in the US will contract CM during his or her lifetime. The various stages of melanoma have been well described, however, to devise improved treatments allowing patients to lead longer and more productive lives, a better understanding of progression of CM is needed. The purpose of this proposal is to produce highly differentiated, tissue culture models of the various stages of CM progression. During Phase I, cell lines from the various disease states of CM will be expanded in monolayer culture and characterized. Using a serum free culture system, these cells will be incorporated into an organotypic, full thickness skin model to produce tissue reconstructs which parallel the various stages of CM in vivo. The tissues will be characterized in terms of morphology, tumor progression, and adhesion receptors. Finally, reproducibility of the tissue models will be investigated.