Human Tissue Model For Heterosexual HIV Transmission

异性艾滋病毒传播的人体组织模型

• 批准号: 6626084
• 负责人: MITCHELL KLAUSNER
• 金额: $ 53.96万
• 依托单位: MATTEK CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626084
• 关键词: HIV infections; Langerhans' cell; cervix; clinical research; communicable disease transmission; disease /disorder model; heterosexuals; histology; human tissue; immunocytochemistry; method development; model design /development; postmortem; tissue /cell culture; transmission electron microscopy; vagina; virus infection mechanism

DESCRIPTION: (Provided by Applicant) Phase I research adapted primary culture methods to obtain a reliable source of normal ectocervico-vaginal (ECV) epithelial and Langerhans precursor (LP) cells. Tissue culture conditions were developed to induce these cells to differentiate into a three-dimensional, organotypic tissue. Characterization showed that the tissue reproduced many of the histological, ultrastructural, and protein expression properties of native tissue. Initial experiments demonstrated the utility of the tissue model for predicting ECV irritation and for HIV infection/ transmission studies. Finally, an economic assessment of the costs involved in producing such tissues showed that the ECV tissue product appears to be commercially feasible. The Phase II work plan will expand upon the results of Phase I to further develop the ECV tissue model. Culture conditions will be optimized and the tissue will be thoroughly characterized. Protocols for irritation studies will be finalized and the mechanisms of HIV infection and transmission in the tissue will be delineated. Finally, methodology for testing HIV microbicides will be developed.

描述：（申请人提供）I期研究适应原代培养 获得正常子宫颈-阴道（ECV）可靠来源的方法 上皮和朗格汉斯前体（LP）细胞。组织培养条件为 诱导这些细胞分化成三维的， 器官型组织表征表明，该组织复制了许多 组织学、超微结构和蛋白质表达特性， 组织.最初的实验证明了组织模型的实用性， 预测ECV刺激和HIV感染/传播研究。最后， 对生产这种组织所涉及的成本进行的经济评估表明， ECV组织产品似乎具有商业可行性。II期 工作计划将在第一阶段成果的基础上进一步发展ECV 组织模型将优化培养条件， 彻底表征。刺激性研究方案将最终确定 艾滋病病毒在组织中的感染和传播机制将被 描绘的。最后，测试艾滋病毒杀微生物剂的方法将 开发