In Vitro Skin Model for Percutaneous Absorption Studies

用于经皮吸收研究的体外皮肤模型

• 批准号: 6481054
• 负责人: MITCHELL KLAUSNER
• 金额: $ 18.14万
• 依托单位: MATTEK CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-17 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6481054
• 关键词: ascorbate; biological models; ceramides; collagen; fibroblasts; growth media; high performance liquid chromatography; histogenesis; histology; human tissue; humidity; hydropathy; keratinocyte; lipids; model design /development; paracrine; skin; skin absorption; tissue /cell culture; transdermal drug delivery; transmission electron microscopy

DESCRIPTION (provided by the applicant): The ability to produce skin in vitro with barrier properties equivalent to those of native epidermis remains a much sought after goal. The current proposal will utilize a number of recent improvements in the organotypic culture of skin cells to optimize the barrier properties of a highly differentiated, serum-free skin model. Medium additives, methods of culture, and the original cell source from which the epidermal cells were derived will be investigated in order to reconstruct in vivo-like skin. The modified skin tissues will be characterized in terms of histology, surface hydration, lipid content, and ultrastructure. Finally, the permeability characteristics of the in vitro tissues in relation to three model drugs spanning a range of lipophilicity will be investigated. PROPOSED COMMERCIAL APPLICATION: The improved skin tissue resulting from this research will find utilization in transdermal drug delivery studies, risk assessment related to accidental or occupational exposure of chemicals to the skin, and skin irritation studies. In addition, the methodology developed will likely improve cultured skin graft material since it has ben reported that skin tissue with improved barrier properties increases the efficacy of such grafts.

描述（由申请方提供）：体外生成皮肤的能力 具有与天然表皮相当的屏障性能， 追求目标。目前的建议将利用一些最近的 改进皮肤细胞的器官型培养以优化屏障 高度分化的无血清皮肤模型的特性。中添加剂， 培养方法，以及表皮细胞的原始细胞来源 将被研究，以重建体内类似的皮肤。 修饰的皮肤组织将在组织学、表面形态学和生物学特性方面进行表征。 水化、脂质含量和超微结构。最后，渗透性 与三种模型药物相关的体外组织特征 将研究跨越亲脂性范围的化合物。 拟定商业应用：从这项研究中得到的改善的皮肤组织将在透皮给药研究、与化学品对皮肤的意外或职业暴露相关的风险评估以及皮肤刺激研究中得到利用。 此外，所开发的方法可能会改善培养的皮肤移植材料，因为据报道，具有改善的屏障性能的皮肤组织增加了这种移植物的功效。