Reconstructed Human Oral Tissues

描述（由申请人提供）：I期研究采用原代培养方法获得正常人颊上皮（NHBE）细胞的可靠来源。采用组织培养的方法诱导NHBE细胞向三维器官型组织分化。表征表明，该组织复制了许多天然颊组织的组织学，蛋白质表达和屏障功能特性。最初的实验研究了使用NHBE组织预测口腔刺激和经口腔给药率。最后，对组织生产成本的经济评估表明，NHBE产品在商业上是可行的。

DESCRIPTION (provided by applicant): Phase I research adapted primary culture methods to obtain a reliable source of normal human buccal epithelial (NHBE) cells. Tissue culture methods were developed to induce the NHBE cells to differentiate into a three-dimensional organotypic tissue. Characterization showed that the tissue reproduced many of the histological, protein expression, and barrier function properties of native buccal tissue. Initial experiments investigated use of the NHBE tissue for predicting oral irritation and transbuccal drug delivery rates. Finally, an economic evaluation of costs involved in tissue production showed that an NHBE product appears to be commercially feasible. The Phase II work plan will expand upon the results of Phase I to further develop the NHBE and other related, and more complex, oral mucosal models. NHBE culture conditions will be optimized and conditions to produce a gingival phenotype and incorporate lamina propria will be determined. Protocols to predict oral irritation will be determined and use of the tissue for transbuccal drug delivery will be studied in a more comprehensive manner. Finally, studies addressing basic oral biology phenomena, namely anti-microbial peptide regulation and HIV infection, will be performed.