CORE--CALCIUM ABSORPTION AND BIOCHEMISTRY

核心--钙吸收与生物化学

• 批准号: 6200962
• 负责人: MUNRO PEACOCK
• 金额: $ 24.16万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6200962
• 关键词: 1,25 dihydroxycholecalciferol; 25 hydroxycholecalciferol; acid phosphatase; alkaline phosphatase; androstenedione; biomarker; biomedical facility; bone density; bone metabolism; calcium metabolism; clinical chemistry; dehydroepiandrosterone; dietary calcium; estradiol; human subject; insulinlike growth factor; normal ossification; nutrition related tag; osteocalcin; parathyroid hormones; pathologic bone resorption; photon absorptiometry; sex hormones; somatotropin; testosterone

The primary goal is to support the subprojects by supplying precise and accurate biochemical measurements of markers of bone formation and bone resorption, calcium-regulating hormones, calcium absorption, sex steroids, and IGF and growth hormone. Bone formation will be assessed by serum osteocalcin and serum bone alkaline phosphatase. Bone resorption will be assessed by serum tartrate resistant acid phosphatase, urinary deoxypyridinoline and urinary n-telopeptide crosslinks. Calcium-regulating hormones will be assessed by serum parathyroid hormone, serum 250H vitamin D, and serum 1,25(OH)2 vitamin D. Calcium absorption will be assessed from the specific activity in serum following an oral dose of stable calcium labeled with radiocalcium. The sex steroids will be assessed by serum estradiol, estrone sulphate, estrone, testosterone, androstenedione, dehydroepiandrosterone and dehydroepiandrosterone sulphate, and sex- hormone binding globulin. Growth hormone will be assessed by measurements of urinary growth hormone and serum IGF and IGF-BP3. The biochemistry core will also be responsible for ensuring that all biological samples are logged-in, processed and stored under optimal conditions; that assays are performed with precision and accuracy; that the data generated are entered in the biochemistry database and transferred in a timely fashion to the biostatistical core for merging with clinical variables for analysis.

主要目标是通过提供精确的、 骨形成和骨形成标志物的准确生物化学测量 再吸收，钙调节激素，钙吸收，性类固醇， IGF和生长激素。将通过血清评估骨形成 骨钙素和血清骨碱性磷酸酶。骨吸收将是 通过血清抗酒石酸酸性磷酸酶、尿 脱氧吡啶啉和尿N-端肽交联。钙调节 激素将通过血清甲状旁腺激素、血清250 H维生素 D和血清1，25（OH）2维生素D。钙吸收将从 口服稳定钙后血清中的比活度 用放射性钙标记。性类固醇将通过血清进行评估 雌二醇，硫酸雌酮，雌酮，睾酮，雄烯二酮， 脱氢表雄酮和脱氢表雄酮硫酸盐，以及性别- 激素结合球蛋白将通过测量来评估生长激素 尿生长激素和血清IGF和IGF-BP 3。 生物化学核心还将负责确保所有 生物样本在最佳条件下登录、处理和储存 条件;测定的精密度和准确度; 所产生的数据被输入到生物化学数据库中， 及时转移到生物统计核心进行合并 临床变量进行分析。