CORE--CALCIUM ABSORPTION AND BIOCHEMISTRY

主要目标是通过提供准确的和 骨形成和骨形成标志物的准确生化测量 吸收，钙调节激素，钙吸收，性类固醇， 胰岛素样生长因子和生长激素。骨形成将通过血清进行评估 骨钙素和血清骨碱性磷酸酶。骨吸收将是 用血清抗酒石酸酸性磷酸酶、尿液进行评估 脱氧吡啶酚和尿n-端肽交联物。钙调节 激素将通过血清甲状旁腺激素、血清250小时维生素进行评估 D，和血清1，25(OH)2维生素D。钙的吸收将从 口服稳定钙后的血清比活度 用放射性钙标记的。性激素将通过血清进行评估。 雌二醇，硫酸雌酮，雌酮，睾酮，雄烯二酮， 脱氢表雄酮和脱氢表雄酮硫酸盐，以及性别- 激素结合球蛋白。生长激素将通过测量进行评估 尿生长激素和血清IGF、IGF-BP3。 生化核心还将负责确保所有 生物样本在最佳条件下登录、处理和存储 条件；以精确和准确的方式进行化验； 生成的数据被输入到生化数据库中，并且 及时转移到生物统计核心进行合并 用临床变量进行分析。

The primary goal is to support the subprojects by supplying precise and accurate biochemical measurements of markers of bone formation and bone resorption, calcium-regulating hormones, calcium absorption, sex steroids, and IGF and growth hormone. Bone formation will be assessed by serum osteocalcin and serum bone alkaline phosphatase. Bone resorption will be assessed by serum tartrate resistant acid phosphatase, urinary deoxypyridinoline and urinary n-telopeptide crosslinks. Calcium-regulating hormones will be assessed by serum parathyroid hormone, serum 250H vitamin D, and serum 1,25(OH)2 vitamin D. Calcium absorption will be assessed from the specific activity in serum following an oral dose of stable calcium labeled with radiocalcium. The sex steroids will be assessed by serum estradiol, estrone sulphate, estrone, testosterone, androstenedione, dehydroepiandrosterone and dehydroepiandrosterone sulphate, and sex- hormone binding globulin. Growth hormone will be assessed by measurements of urinary growth hormone and serum IGF and IGF-BP3. The biochemistry core will also be responsible for ensuring that all biological samples are logged-in, processed and stored under optimal conditions; that assays are performed with precision and accuracy; that the data generated are entered in the biochemistry database and transferred in a timely fashion to the biostatistical core for merging with clinical variables for analysis.