GENETICS OF BONE LOSS AT THE HIP IN WOMEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 髋部骨量的遗传力很高，但髋部骨丢失的遗传力尚未被研究。对760对姐妹的骨强度遗传力进行了测量，一个10 cM的基因组筛查发现了几个影响骨强度的染色体位点。将要检验的假设是，女性髋部的骨丢失率是由基因决定的。这将通过重新测量至少5年前参加过GCRC 578议定书的500对姐妹的髋部骨量来实现。采用LUNAR DPX-L双X线骨密度仪测量右股骨颈、股骨粗隆及Ward、腰椎L2~L4和全身。下骨盆和股骨上端15度内旋转的X光照片将在标准的X光设备上拍摄。所有的结构测量都将在同一个髋关节上使用数字卡尺进行。将根据已建立的方案进行体积定量计算地形图(CT)。采集空腹血和尿液，用于钙调节激素和骨转换标志物的测定。人体测量将在专用仪器上收集，整体瘦身和脂肪由DXA测量。 问卷将包括个人、家庭和病史、妇科病史、服药史、吸烟、体力活动和饮食。所有问卷都得到了核实，并在过去五年中一直在使用，在目前的姊妹研究中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heritability of bone mass at the hip is high, but heritability of bone loss at the hip has not been studied. Heritability of bone strength has been measured in 760 pairs of sisters, and a 10cM genome screen has identified several chromosomal loci influencing strength. The hypotheses that will be tested is that the rate of bone loss at the hip in women is genetically determined. This will be achieved by remeasuring hip bone mass in 500 pairs of sisters that had participated in the GCRC Protocol 578 a minimum of 5 years ago. Using the Lunar DPX-L dual X-ray absorptiometry (DXA) the following sites will be measured: the right femoral neck, trochanter and wards, lumbar vertebrae L2 to L4 and total body. Radiographs of the lower pelvis with upper femura in 15 degree internal rotation will be taken on standard X-ray equipment. All structural measurements will be made on the same hip using a digital caliper. Volumetric Quantitative Computed Topograph (CT) will be performed according to an established protocol. Fasting blood and urine will be collected for measurement of calcium regulating hormones and bone turnover markers. Anthropometrics will be collected on dedicated instruments, total body lean and fat are measured by DXA. Questionnaires will be administered which include personal, family and medical history, gynecological history, medication history, smoking, physical activity and diet. All questionnaires have been verified and have been in use over the last five years in the current sister study.