GENETICS OF BONE LOSS AT THE HIP IN WOMEN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 髋部骨量的遗传率很高，但髋部骨丢失的遗传率尚未研究。在760对姐妹中测量了骨强度的遗传性，并且10cM基因组筛选已经确定了影响强度的几个染色体位点。将要检验的假设是，女性髋部骨丢失率是由遗传决定的。这将通过重新测量至少5年前参与GCRC方案578的500对姐妹的髋部骨量来实现。将使用Lunar DPX-L双X线吸收测定仪（DXA）测量以下部位：右股骨颈、转子和Wards、腰椎L2至L4和全身。将在标准X射线设备上拍摄下骨盆和上股骨15度内旋的X线片。所有结构测量将使用数字卡尺在同一髋关节上进行。将根据既定方案进行体积定量计算机断层扫描（CT）。将采集空腹血液和尿液，用于测量钙调节激素和骨转换标志物。将在专用仪器上收集人体测量数据，通过DXA测量全身瘦体重和脂肪。 将管理包括个人、家族和病史、妇科史、用药史、吸烟、体力活动和饮食的调查表。所有调查问卷均经过验证，并已在当前姐妹研究中使用了五年。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heritability of bone mass at the hip is high, but heritability of bone loss at the hip has not been studied. Heritability of bone strength has been measured in 760 pairs of sisters, and a 10cM genome screen has identified several chromosomal loci influencing strength. The hypotheses that will be tested is that the rate of bone loss at the hip in women is genetically determined. This will be achieved by remeasuring hip bone mass in 500 pairs of sisters that had participated in the GCRC Protocol 578 a minimum of 5 years ago. Using the Lunar DPX-L dual X-ray absorptiometry (DXA) the following sites will be measured: the right femoral neck, trochanter and wards, lumbar vertebrae L2 to L4 and total body. Radiographs of the lower pelvis with upper femura in 15 degree internal rotation will be taken on standard X-ray equipment. All structural measurements will be made on the same hip using a digital caliper. Volumetric Quantitative Computed Topograph (CT) will be performed according to an established protocol. Fasting blood and urine will be collected for measurement of calcium regulating hormones and bone turnover markers. Anthropometrics will be collected on dedicated instruments, total body lean and fat are measured by DXA. Questionnaires will be administered which include personal, family and medical history, gynecological history, medication history, smoking, physical activity and diet. All questionnaires have been verified and have been in use over the last five years in the current sister study.