METALLO-BIOCHEMISTRY

由于它们的光谱和化学性质， 镧系元素(III)离子是钙的重要光谱探针 以及生物系统中的镁结合研究。例如，欧盟(III) 发光研究已经成为衡量细胞数量的重要指标 生化物质与水分子相互作用的原子和水分子 金属离子区域。然而，尽管他们有二十多年的历史 在生物系统中的应用，关于生物系统的许多基本信息 这些稀土离子与其周围环境的相互作用仍然是 未知。我们建议使用光谱技术，包括超声波 吸收、FT-核磁共振、FT-IR和UV-VIS方法，以开发关于 这些阳离子的内部配位壳层的组成。一个 这项建议的第二个方面是研究d-过渡离子、钴、 镍、铜和锌，在水和混合溶剂中，了解 围绕金属离子中心的区域。具体研究包括：(1) 用硫酸盐超声吸收Yb(III)，用18-冠-6超声吸收La(III)， 和锌(II)与甘氨酸和可能的碳酸氢酶或 羧肽酶A(2)钴(II)平衡常数的测定 与氯离子和甘氨酸，镍(II)和铜(II)与SCN-，以及铜(II)与 硫酸盐。(3)有机溶剂化LaCl/3的结构研究 用傅立叶变换核磁共振和傅立叶变换红外光谱在低温下测定有机溶剂的含量 内部协调壳的组成。(4)锌的傅里叶变换核磁共振研究 混合溶剂中的络合物，以检测溶液中的几何变化。(5) 我们打算开始比较镧系元素和鳗系元素。 关于生物和环境问题未来工作的要素 关于鳗系元素污染的事。(6)调查复杂地层 在巴克明斯特富勒烯和有机给体/受体分子之间。

Because of their spectroscopic and chemical properties, the lanthanide(III) ions have been important spectroscopic probes for calcium and magnesium binding studies in biological systems. For example, Eu(III) luminescence studies have become an important measure of the number of interacting atoms and water molecules between a biochemical material and the metal ion domain. However, despite over twenty years of their applications in biological systems, much fundamental information about the interactions of these lanthanide ions with their surroundings is still unknown. We propose using spectroscopic techniques, including ultrasonic absorption, FT-NMR, FT-IR and UV-vis methods, to develop information about the composition of the inner coordination shell of these cations. A second aspect of this proposal is the study of d-transition ions, cobalt, nickel, copper and zinc, in water and mixed solvents to learn about the domains surrounding the metal ion centers. Specific studies include: (1) Ultrasonic absorption of Yb(III) with sulfate, La(III) with 18-crown-6, and Zn(II) with glycine and possibly carbonic anhydrase or carboxypeptidase A. (2) Equilibrium constant determinations of Co(II) with Cl- and with glycine, Ni(II) and Cu(II) with SCN-, and Cu(II) with sulfate. (3) Structural studies of solvated LaCl/3 in aqueous organic solvents using FT-NMR and FT-IR at low temperatures to determine the composition of the inner coordination shell. (4) FT-NMR studies of zinc complexes in mixed solvents to detect geometry changes in solution. (5) We intend to initiate comparisons between the lanthanides and the actinide elements towards future work on biological and environmental concerns about actinide contamination. (6) We will investigate complex formation between the buckminsterfullerenes and organic donor/acceptor molecules.