P02 HISTOGRAPH 6650

该提案要求购买Eppendorf仪器， 使用基于自动化技术测量组织中的PO 2 专门研发的微型克拉克电极我们要求该设备用于 几个相互关联的目的。它现在几乎已经成为强制性的， 为了使用该装置进行组织P02的主要测量 用它来比较临床和实验室组织中p02的测量值， 其他方法进行的动物实验，包括常规方法 微电极，因为Eppendorf装置已迅速成为 “黄金标准”的测量。尤为重要 使用EPR血氧测定法等新技术进行相关测量 技术，以便有充分的可信度， 新技术的能力;这包括对 两种技术采样的潜在不同域。的高度进化的 Eppendorf装置的构造使其成为克拉克电极， 系统的选择，用于几种类型的测量，包括： 因为肌肉和肝脏， 由于电极的易碎性 提示：对于低p02值，例如发生在肿瘤和缺血中， 其它商业电极的通常尺寸消耗太多的氧; 这是商业工具的独特能力： 沿沿着轨道执行高度受控的测量模式，使用 程序步骤和内部重新校准。Eppendorf系统是 只有商业仪器具有必要的灵敏度， 耐用性和基本组件的可用性。尽管其 相对较高的价格，它将是较便宜的获得和维护 一个自我构建的系统， 生产类似的自制氧电极需要多年的时间， 经验和其他功能的增加，如 电极尖端的受控穿透和坚固的结构 会大大增加时间开发我们自己的同类产品 “Eppendorf”将把关键的精力从新的研究转移到 试图复制现有的技术。在该机构内， 我们特别指出Eppendorf仪器的可用性 将大大促进所有四个主要用户的进展， 在这个提案中合作。我们已经包括了足够的额外的 组件，以使其具有最大的效率， 该设备的多个用户的有效性。

This proposal requests the purchase of the Eppendorf apparatus for measuring the p02 in tissues using an automated technique based on a specially developed micro Clark electrode. We request this apparatus for several inter-related purposes. It now has become almost mandatory either to use this apparatus for the principal measurements of tissue p02 or to use it to compare measurements of p02 in tissues for both clinical and animal experiments made by other methods, including conventional microelectrodes, because the Eppendorf apparatus has rapidly become the "gold standard" for such measurements. It is especially important to relate measurements made with new technologies such as EPR oximetry techniques in order to have full credibility for the additional capabilities of the new technique; this includes considerations of the potential different domains sampled by two techniques. The highly evolved construction of the Eppendorf apparatus makes it the Clark electrode system of choice for several types of measurements including: tissues such as muscle and liver for which measurements with conventional Clark electrodes are very different because of the fragility of the electrode tips; for low p02 values such as occur in tumors and in ischemia because the usual size of other commercial electrodes consumes too much oxygen; the capability that is unique in commercial instruments: the ability to carry out a highly controlled pattern of measurements along a track, using programmed steps and internal recalibrations. The Eppendorf system is the only available commercial instrument with the requisite sensitivity, ruggedness, and availability of essential components. In spite of its relatively high price, it would be less expensive to obtain and maintain that a self-constructed system because the attainment of the capabilities to produce comparable homemade oxygen electrodes requires many years of experience and the addition of the other capabilities such as the controlled penetration of the electrode tip and the rugged construction would add significantly to that time. Development of our own equivalent "Eppendorf" would divert critical effort away from new research into attempts to reproduce established technology. Within the body the proposal we indicate specifically how the availability of the Eppendorf apparatus will greatly facilitate the progress of all four of the major users who have collaborated in this proposal. We have included sufficient additional components to make it feasible to have maximal efficiency and effectiveness with multiple users of this apparatus.