CORE-SCIENTIFIC

科学核心的存在是为了促进科学项目， 节目 提供的服务、材料和试剂 科学核心是纯化的羊瘙痒症朊病毒，PrP抗体，合成 肽和寡核苷酸，重组PrP变体，转基因小鼠 筛选和化学及生化分析服务。 这一核心 将由Michael Baldwin博士指导，他是一位分析化学家， 拥有丰富的经验，曾领导过核心服务实验室 他在伦敦大学任教14年， 在过去的六年里， 蛋白 纯化朊病毒的制备已在本实验室进行。 实验室超过十年。 近年来，该计划已 由哈娜·塞尔班女士监督 她将监督PrP的生产 抗体，对项目1 - 4和核心C的成功至关重要。 两 SRA的，都有相当丰富的经验，将合成肽， 寡核苷酸 项目3 - 5需要肽， 抗体生产。 项目3将需要寡核苷酸， 4和该核心用于转基因小鼠生产和用于筛选这些 以及为分子生物学提供各种其他工具。 重组PrP变体将由Michael博士在培养物中表达 斯科特，一位杰出的分子生物学家，用于项目4和5。 项目3所必需的转基因小鼠的筛选将 由露丝·科勒女士负责 一个SRA将进行化学和 生化分析，这将发挥重要作用，抗体 生产和转基因小鼠的生产，也将有利于项目 2-5. 所有这些活动目前都在非常积极地进行。 在这个实验室里取得了成功， 人员已经到位。

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified scrapie prions, PrP antibodies, synthetic peptides and oligonucleotides, recombinant PrP variants, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Dr. Michael Baldwin, an analytical chemist of considerable experience who directed a core service laboratory in the University of London for 14 years and who has devoted himself for the last six years to reach into the chemistry and biochemistry of the prion protein. The preparation of purified prions has been carried out in this laboratory for more than a decade. In recent years this program has been supervised by Ms. Hana Serban. She will supervise the production of PrP antibodies, crucial to the success of projects 1-4 and Core C. Two SRA's, both having considerable experience, will synthesize peptides and oligonucleotides. Peptides are required for projects 3-5, and for antibody production. oligonucleotides will be needed for projects 3 and 4 and this core for transgenic mouse production and for screening these mice as well as providing a variety of other tools for molecular biology. Recombinant PrP variants will be expressed in culture by Dr. Michael Scott, an outstanding molecular biologist, for use in projects 4 and 5. The screening of transgenic mice which is essential for project 3 will be carried by Ms. Ruth Koehler. An SRA will carry out chemical and biochemical analysis, which will play an important role in antibody production and transgenic mouse production and will also benefit projects 2-5. All of these activities are currently being carried out very successfully in this laboratory and there are highly experienced personnel already in post.