CORE--SCIENTIFIC

科学核心的存在是为了促进科学项目， 节目 提供的服务、材料和试剂 科学核心是纯化的羊瘙痒症朊病毒，PrPs抗体，合成 肽和寡核苷酸，以及肽和 proteins. 这个核心将由迈克尔鲍德温博士， 一位经验丰富的分析化学家，他指导了一项核心服务， 他在伦敦大学的实验室工作了14年， 他自己研究朊病毒化学和生物化学， 4年 纯化的朊病毒的制备已经在此进行， 实验室十多年了。 近年来，该计划已 由哈娜·塞尔班女士监督 她将监督2个SRA II的工作 谁将进行项目所需的大规模净化 II和III以及用于抗体生产。 PrP抗体，对于 这些项目中的大多数项目的成功，将由 另一个SRAII。 进一步的SRA II/III将合成肽， 寡核苷酸;任命一个新的人担任这一职务是 必须科普大量的工作，将创造 这个戒酒会这 肽是抗体产生和免疫调节所必需的。 各种其他目的，包括结合研究、结构分析 和用于产生重组Fab的活性位点/感染性， 在项目IV中，为生产转基因小鼠建造实验室 和筛选这些小鼠。 肽和蛋白质分析也将是 由另一个SRAII执行。 所有这些活动目前 在这个实验室里进行得非常成功， 经验丰富的工作人员已经在岗位上。

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified scrapie prions, PrPs antibodies, synthetic peptides and oligonucleotides, and an analytical service for peptides and proteins. This core will be directed by Dr. Michael Baldwin, an analytical chemist of considerable experience who directed a core service laboratory in the University of London for 14 years and who has devoted himself to research into prion chemistry and biochemistry for the last 4 years. the preparation of purified prions has been carried out in this laboratory for more that a decade. In recent years this program has been supervised by Ms. Hana Serban. She will oversee the work of 2 SRA II's who will carry out the large-scale purifications required for projects II and III and for antibody production. PrP antibodies, crucial to the success of most of these projects, will be prepared and supplied by another SRAII. A further SRA II/III will synthesize peptides and oligonucleotides; the appointment of a new person to this post is essential to cope with the high volume of work that will be created by this program. Peptides are required for antibody production and for a variety of other purposes including binding studies, structural analysis and active site/infectivity for the production of recombinant Fab's and in project IV for construction of cosmids for transgenic mouse production and for screening these mice. Peptide and protein analysis will also be carried out by a further SRAII. All of these activities are currently being carried out very successfully in this laboratory and there are highly experienced personnel already in post.