DVMT OF MASS SPECTROMETRY AS TOOL FOR XRAY CRYSTALLOGRAPHIC STUDIES OF PROTEINS

我们继续开发质谱（MS）的实用性， 利用x射线促进蛋白质的原子分辨率研究 衍射和NMR光谱。 我们已经发现MS是巨大的 在这些研究的每个阶段都很有用，包括： 1)确定 重组蛋白构建体的纯度和保真度。 （二） 阐明紧密折叠的结构域以协助设计 最佳蛋白质结构。 3)评估重原子掺入 4)评估共晶中多种组分的化学计量。 进展继续保持稳定，我们正在准备 另一份手稿描述了我们较新的方法论（这将是 对结构生物学家有很大的用处）。

We continue to develop the utility of mass spectrometry (MS) for facilitating atomic resolution studies of proteins by x-ray diffraction and NMR spectroscopy. We have found that MS is enormously useful at every stage of these studies, including:. 1) Determining the purity and fidelity of the recombinant protein constructs . 2) Elucidating tightly folded structural domains to assist in the design of optimal protein constructs. 3) Assessing heavy atom incorporation 4) Assessing the stochiometry of multiple components in co-crystals. Progress continues to be steady and we are getting ready to prepare another manuscript describing our newer methologies (which will be of considerable use to structural biologists).