PROTEIN PROTEIN & PROTEIN DNA INTERACTIONS STUDIED BY CHEMICAL CROSS LINKING

这项调查的目的是使用化学物质 与质量一起发生的交联反应和化学改性 用光谱学技术研究分子间的接触作用 蛋白质-蛋白质和蛋白质-DNA复合体。各种交联剂和 在这些研究中选择了改性剂。 探测多种氨基酸可及性的试剂 已对侧链进行了调查，包括他的、Try、Lys和Arg。 我们的研究表明，激光解吸质谱在 遵循反应的性质和程度。我们目前正在 开发使用MALDI离子陷阱来绘制 使用CAD-MS/MS进行修改。

The objective of this investigation is to use chemical cross-linking reactions and chemical modification together with mass spectrometric techniques to study the contact interactions between protein-protein and protein-DNA complexes. Various cross-linking and modification reagents have been selected for these investigations. Reagents that probe the accessibilities of a number of amino acid side-chains have been investigated, including His, Try, Lys, and Arg. Our studies have shown that laser desorption MS is very useful in following the nature and extent of reaction. We are currently developing the use of the MALDI-Ion Trap to map the sites of modifications with CAD-MS/MS.