SENSITIVE ENHANCEMENT OF MALDI TOF MS

我们已经比较了我们内部建造的 延迟提取线阵MALDI-TOF仪器 商用PerSeptive STR MALDI-TOF仪器 灵敏度和质谱图质量。在线性模式下，我们的内部 构造的文书似乎大约是一个数量级的 比商业仪器更灵敏的幅度(I结局 多肽的数量给出信噪比过高的信号 在内部构造的设备中为100：1)。商业广告 仪器给出了非常高质量的-10fmol量的光谱 反射模式中的多肽。我们继续实施改进措施 我们相信商业仪器最终将导致 以进一步增强性能。

We have compared the performance of our in-house constructed delayed-extraction linear MALDI-TOF instrument with our newly acquired commercial PerSeptive STR MALDI-TOF instrument with respect to sensitivity and mass spectrum quality. In linear mode, our in-house constructed instrument appears to be approximately one order of magnitude more sensitive than the commercial instrument (I finale amounts of peptide give signals with signal-to-noise ratios in excess of 100: 1 in the in-house constructed device). The commercial instrument gives very high quality spectra for -10fmol amounts of peptide in the reflecting mode. We continue to implement improvements to the commercial instrumentation that we believe will ultimately lead to further enhancements in performance.