DEUTERIUM OXIDE ENHANCES FLUORESCENCE INTENSITY & LIFETIME OF DNA PROBES

光谱分析与常规流动和相敏流动 用流式细胞术比较PI和EB荧光的变化 DNA和固定的中国仓鼠卵巢的结合强度和寿命 (CHO)细胞在D2O存在下与磷酸盐缓冲盐水的比较 (PBS)。PI和EB的荧光强度提高了一倍 在D2O中注意到与固定的CHO细胞结合以及5 ns的增加 在PI和EB中荧光寿命在D2O中。凋亡亚群 的HL-60细胞的荧光寿命显著缩短 与非凋亡性亚群相比。结果表明， 不同的染色质状态，或PI和 EB导致荧光强度和荧光强度的变化 这些嵌入探头的寿命。

Spectroscopic analysis and conventional and phase-sensitive flow cytometry were used to compare changes in PI and EB fluorescence intensity and lifetime bound to DNA and fixed Chinese hamster ovary (CHO) cells in the presence of D2O versus phosphate buffered saline (PBS). A two-fold enhancement of fluorescence intensity of PI and EB bound to fixed-CHO cells in D2O was noted as well as a 5 ns increase in PI and EB fluorescence lifetimes in D2O. Apoptotic sub-populations of HL-60 cells had a significantly reduced fluorescence lifetime compared to non-apoptotic sub-populations. Results indicate that different chromatin states, or differences in the structures of PI and EB lead to alterations in the fluorescence intensity and fluorescence lifetime of these intercalating probes.