APOPTOSIS DIFFERENCES FOR ETHIDIUM BROMIDE BOUND TO RESIDUAL CHROMATIN IN CELLS

荧光寿命分析与 常规流式细胞术分析，以监测残留 凋亡HL-60细胞群中的染色质 喜树碱、放线菌酮、染料木黄酮、H7和γ辐射。 数据 显示所有这些代谢抑制剂通过不同的途径起作用， 信号级联，产生凋亡亚群， 但DNA结合的溴化乙锭（EB）的寿命不同。 结果 结合常规流程，指示寿命分析 流式细胞术，可用于早期检测糖尿病诱导的 染色质的变化，也可能提供新的信息， 不同凋亡诱导剂的作用。

Fluorescence lifetime analysis was used in combination with conventional flow cytometric analysis to monitor changes in residual chromatin in apoptotic HL-60 cell populations following treatment with camptothecin, cycloheximide, genistein, H7, and gamma radiation. Data show that all of these metabolic inhibitors that act through different signaling cascades, produce apoptotic sub-populations with decreased, but different lifetimes for DNA-bound ethidium bromide (EB). Results indicate that lifetime analysis, in conjunction with conventional flow cytometry, can be useful for early detection of apoptosis-induced chromatin changes and can also potentially provide new information on the effects of different apoptosis inducing agents.