DEUTERIUM OXIDE ENHANCES FLUORESCENCE INTENSITY & LIFETIME OF DNA PROBES

光谱分析和常规及相敏流动 用流式细胞术比较PI和EB荧光的变化 与DNA和固定的中国仓鼠卵巢结合的强度和寿命 (CHO)D2 O与磷酸盐缓冲盐水存在下的细胞 （PBS）。 PI和EB的荧光强度增强两倍 与D2 O中固定的CHO细胞结合，并增加5 ns 在D_2O中的PI和EB荧光寿命。 凋亡亚群 HL-60细胞的荧光寿命明显缩短 与非凋亡亚群相比。 结果表明 不同的染色质状态，或PI和 EB导致荧光强度和荧光强度的改变， 这些嵌入探针的寿命。

Spectroscopic analysis and conventional and phase-sensitive flow cytometry were used to compare changes in PI and EB fluorescence intensity and lifetime bound to DNA and fixed Chinese hamster ovary (CHO) cells in the presence of D2O versus phosphate buffered saline (PBS). A two-fold enhancement of fluorescence intensity of PI and EB bound to fixed-CHO cells in D2O was noted as well as a 5 ns increase in PI and EB fluorescence lifetimes in D2O. Apoptotic sub-populations of HL-60 cells had a significantly reduced fluorescence lifetime compared to non-apoptotic sub-populations. Results indicate that different chromatin states, or differences in the structures of PI and EB lead to alterations in the fluorescence intensity and fluorescence lifetime of these intercalating probes.