STRUCTURAL BASIS OF CELL MEMBRANE RECOGNITION & ADHESION

细胞膜识别的结构基础

• 批准号: 6119480
• 负责人: William I Weis
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119480
• 关键词: biomaterials; immunology; inflammation; nervous system; proteins

This program aims to determine the structure of proteins involved in cell membrane recognition, targeting, and adhesion, in order to establish the mechanism and specificity of their interactions. There are three principal areas under investigation. 1) C-type animal lectins are a large familty of Ca2+ dependent carbohydrate-binding proteins. We are studying C-type lectins that function in cell-cell adhesion in leukocyte trafficking (selectins) and others that participate in the innate immune response by recognizing carbohydrate structures present on pathogenic cell surfaces (mannose-binding proteins and the macrophage mannose receptor). 2) Interactions of catenins. Catenins are cytoplasmic proteins that link cadherin cell adhesion molecules to the cytoskeleton. The interactions of catenins are essential to intercellular adhesion in solid tissues, and their assembly in cell junctions is a dynamic and highly regulated process. Beta-catenin is also an essential component of the wnt signalling pathway that governs cell fate choice during embryogenesis. 3) Proteins that participate in the recognition and fusion of intracellular transport vesicles with their target membranes.

该计划旨在确定所涉及的蛋白质的结构 在细胞膜识别、靶向和粘附方面， 建立它们相互作用的机制和特异性。 那里 是正在调查的三个主要领域。 1)C型动物 凝集素是钙离子依赖性碳水化合物结合的一大家族 proteins. 我们正在研究C型凝集素， 粘附在白细胞运输（选择素）和其他， 通过识别碳水化合物参与先天免疫反应 存在于致病细胞表面的结构（甘露糖结合 蛋白质和巨噬细胞甘露糖受体）。 2)相互作用 连环蛋白 连环蛋白是连接钙粘蛋白细胞的细胞质蛋白 粘附分子粘附到细胞骨架上。 连环蛋白的相互作用 是实体组织中细胞间粘附所必需的， 细胞连接中的组装是动态的和高度调节的过程。 β-连环蛋白也是Wnt信号传导的重要组成部分 在胚胎发生过程中控制细胞命运选择的途径。 第三章 参与识别和融合的蛋白质 细胞内运输囊泡与其靶膜。