STRUCTURE & INHIBITION OF HIV 1 INTEGRASE

结构

• 批准号: 6119508
• 负责人: JULIAN CHEN
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119508
• 关键词: AIDS; biomaterials; communicable diseases; enzymes; immunology; inflammation; lymphatic system; structural biology; virus

We are currently attempting to extend the diffraction limit of the above HIV-1 integrase crystal forms, and others, by screening various crystallization additives, inhibitors, substrate, and cryogenic artificial mother liquors. Since diffraction is significantly improved by the use of synchrotron radiation, SSRL time is clearly required for project continuation. The following experiments will be carried out: 1)Screen for conditions which improve the diffraction limit of the HIV-1 integrase crystals. 2)Minimally, collect high resolution data from apo-crystal B grown in PEG with and without inhibitor, and crystal A.

我们目前正试图扩大衍射极限的 上述HIV-1整合酶晶体形式和其他，通过筛选各种 结晶添加剂、抑制剂、基质和低温 人造母液。 由于衍射显著 通过使用同步辐射的改进，SSRL时间显然是 继续项目所需。 以下实验将 进行：1）筛选改善衍射的条件 HIV-1整合酶晶体的极限。 2）最低限度，收集高 来自在PEG中生长的脱辅基晶体B的分辨率数据， 抑制剂和晶体A。