MUCOUS CELL DIFFERENTIATION IN AIRWAY EPITHELIUM

描述：本更新申请旨在调查维生素 A调节气道上皮中的粘液细胞分化。两 提出了粘液细胞分化的阶段。在第一阶段中， 细胞不能分化成粘液细胞， 维生素A.然而，它们可以转化为产生粘蛋白的细胞， 通过显微注射来自分化粘液细胞的RNA。该第一 因此，这一阶段被称为粘液细胞的“感受态”阶段 分化，维生素A可以导致永生化的气道上皮细胞 细胞系变得有能力。在第二个“渐进”阶段， 维生素A诱导粘液细胞分化。吴医生已经确认 1到3个基因来自于导致第1阶段的进行性阶段细胞 细胞表达粘蛋白。这项建议旨在克隆和 描述这些基因的特征，以确定维生素D A（即，转录或翻译），以阐明 这些基因在气道上皮中的表达，并最终确定其机制。 这些基因通过其诱导粘液细胞分化。

DESCRIPTION: This renewal application seeks to investigate how vitamin A regulates mucous cell differentiation in airway epithelium. Two stages of mucous cell differentiation are proposed. In the first stage, cells are unable to differentiate into mucous cells in response to vitamin A. However, they can be transformed into mucin-producing cells by microinjection of RNA from differentiated mucous cells. This first stage is therefore termed the "competent" stage of mucous cell differentiation, and vitamin A can cause immortalized airway epithelial cell lines to become competent. In the second "progressive" stage, vitamin A induces mucous cell differentiation. Dr. Wu has identified one to three genes from progressive stage cells which cause stage 1 cells to express mucins. This proposal seeks to clone and to characterize these genes, to define the level of regulation by vitamin A (i.e., transcription or translation), to elucidate the expression of these genes in airway epithelium, and finally to define the mechanism by which these genes induce mucous cell differentiation.