NON INVASIVE BLOOD GLUCOSE MONITORING: NADH AUTOFLUORESCENCE SIGNALS FROM EYE

非侵入式血糖监测：来自眼睛的 NADH 自体荧光信号

• 批准号: 6206137
• 负责人: MICHAEL LEVENE
• 金额: $ 0.59万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206137
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical equipment development; biomedical resource; human tissue; lasers; minerals; neoplasm /cancer; nervous system; technology /technique

Pulsed infrared excitation was used to induce autofluorescence of the energy-carrying molecules NADH and NADPH in living brain slices from mouse, cultured neonatal rat hippocampal and cortical neurons, and cultured PC12 cells. Levels of (putatively) NADH and NADPH could be increased by bath application of NaCN and decreased by bath application of mitochondrial proton-gradient uncouplers. Spectra of the autofluorescence closely match the solution spectra of NAD(P)H. Diffuse autofluorescence was observed in some sections of the mouse brain, while consistently bright autofluorescence was observed in an unidentified, possibly epithelial, cell-type lining capillaries. Identification of individual neurons or processes in a brain slice was difficult. However, autofluorescence imaging of NAD(P)H promises to be a useful non-invasive indicator of cellular metabolism in neural systems.

脉冲红外激发用于诱导自发荧光， 活体脑切片中的能量载体分子NADH和NADPH 来自小鼠、培养的新生大鼠海马和皮质神经元， 培养PC12细胞。 （puronous）NADH和NADPH水平可以 镀液中加入NaCN会增加，镀液中加入NaCN会降低 线粒体质子梯度解偶联剂的应用。 光谱 自发荧光与NAD（P）H的溶液光谱非常匹配。 在小鼠的某些切片中观察到弥漫性自体荧光 大脑，而一致明亮的自体荧光观察到在一个 未鉴定的，可能是上皮细胞型内衬毛细血管。 在脑切片中识别单个神经元或过程是 难 然而，NAD（P）H的自发荧光成像有望 是神经系统中细胞代谢的有用的非侵入性指标， 系统.