FEASIBILITY OF KHZ FRAME RATE TWO PHOTON MICROSCOPY

一种测量双光子绝对吸收的方法 已经开发了溶液中分子的横截面。 的 由脉冲红外激发诱导的荧光与 由相同激发引起的荧光频率加倍， 使用非线性晶体来获得（， 消除了对量子产率、收集 效率或浓度。 我们已经结合使用了这种方法 与我们的测量的相对方法的分子设计的 Marder和佩里小组在加州理工学院（见测量分子与 大双光子横截面）具有大（值（5000 GM）。 我们对罗丹明B在甲醇溶液中的测定结果与Xu和WebB的结果一致 （1996年）。 大双光子横截面）具有大（值（5000 GM）。 我们对罗丹明B在甲醇中的测量结果与Xu和 Webb（1996年）。 Chris Xu和Watt W. Webb，“双光子测量 分子荧光团的激发截面，数据来自690 至1050 nm”，J. Opt. Soc. Am. B 13（3），481-491（1996）。

A method for measurement of the absolute two-photon absorption cross-section, (, of a molecule in solution has been developed. The ratio of fluorescence induced by pulsed infrared excitation to fluorescence induced by that same excitation doubled in frequency by a non-linear crystal is used to obtain an absolute value for (, eliminating the need for knowledge of the quantum yield, collection efficiency, or concentration. We have used this method in conjunction with our measurements by relative methods of molecules designed by the Marder and Perry groups at Caltech (see Measurement of Molecules with Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Chris Xu and Watt W. Webb, "Measurement of two-photon exitation cross-sections of molecular fluorophores with data from 690 to 1050 nm" in J. Opt. Soc. Am. B 13 (3), 481-491 (1996).