CONFINEMENT OF OPTICAL EXCITATION USING TWO PHOTON EXCITATION NEAR SHARP TIP

建立了膜片钳电容记录系统 分辨率为0.1 fF，可实现高分辨率测量 贴片里的分泌物。我们已经将该系统应用于RBL细胞，a 通常用于研究分泌物的细胞系。虽然我们已经看到 内吞作用(电容向下)，没有胞吐步骤 在分泌过程中观察到。相反，我们看到 电容，可能指示瞬时聚变事件，其中 囊泡只与质膜短暂融合。工作正在进行中 以确认电容尖峰的性质并探索其 依赖于外部钙和其他必要的条件 分泌物

A patch clamp capacitance recording system has been set up with a resolution of 0.1 fF, allowing high resolution measurements of secretion in the patch. We have applied this system to RBL cells, a cell line commonly used for studying secretion. While we have seen endocytosis (downward steps in capacitance), no exocytotic steps have been observed during secretion. Instead, we see spikes in the capacitance, possibly indicating transient fusion events where the vesicle fuses only briefly with the plasma membrane. Work is underway to confirm the nature of the capacitance spikes and explore their dependence on external calcium and other conditions necessary for secretion