CONFINEMENT OF OPTICAL EXCITATION USING TWO PHOTON EXCITATION NEAR SHARP TIP

使用尖头附近的两个光子激发来限制光学激发

• 批准号: 6349407
• 负责人: MICHAEL LEVENE
• 金额: $ 3.24万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349407
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical resource; human tissue; immunology; inflammation; lipids; lymphatic system

A patch clamp capacitance recording system has been set up with a resolution of 0.1 fF, allowing high resolution measurements of secretion in the patch. We have applied this system to RBL cells, a cell line commonly used for studying secretion. While we have seen endocytosis (downward steps in capacitance), no exocytotic steps have been observed during secretion. Instead, we see spikes in the capacitance, possibly indicating transient fusion events where the vesicle fuses only briefly with the plasma membrane. Work is underway to confirm the nature of the capacitance spikes and explore their dependence on external calcium and other conditions necessary for secretion

建立了一种膜片钳式电容记录系统