FLUORESCENCE ENHANCE FROM OPTICAL SCATTERING THIN FILMS & METAL CLAD WAVEGUIDES

虽然 RBL 细胞是研究分泌的常见系统，但现有的 数据似乎表明质膜面积没有变化， 与许多其他分泌细胞的结果相反。 然而，数据基于全细胞电容测量 （在已发现RBL细胞不分泌的条件下）， 以及细胞的连续电子显微镜重建（这是一个 技术精度不如电容测量）。 我们建议 对 RBL 细胞进行透化贴片电容记录，从而 允许电进入整个细胞膜，同时 维持分泌所需的细胞质完整性。

While RBL cells are common system for studying secretion, existing data seems to indicate that the plasma membrane area does not change, contrary to the results in a number of other secretory cells. However, the data are based on whole cell capacitance measurements (under conditions in which RBL cells have been found not to secrete), and on serial electron microscopy reconstruction of cells (which is a less accurate technique than capacitance measurements). We propose to do permeabilized patch capacitance recording of RBL cells, thus allowing electrical access to the whole cell membrane while maintaining the cytoplasmic integrity necessary to have secretion.