FLUORESCENCE ENHANCE FROM OPTICAL SCATTERING THIN FILMS & METAL CLAD WAVEGUIDES

光学散射薄膜的荧光增强

• 批准号: 6349406
• 负责人: MICHAEL LEVENE
• 金额: $ 2.84万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349406
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical resource; human tissue; immunology; inflammation; lipids; lymphatic system

While RBL cells are common system for studying secretion, existing data seems to indicate that the plasma membrane area does not change, contrary to the results in a number of other secretory cells. However, the data are based on whole cell capacitance measurements (under conditions in which RBL cells have been found not to secrete), and on serial electron microscopy reconstruction of cells (which is a less accurate technique than capacitance measurements). We propose to do permeabilized patch capacitance recording of RBL cells, thus allowing electrical access to the whole cell membrane while maintaining the cytoplasmic integrity necessary to have secretion.

RBL细胞是研究分泌的常用系统， 数据似乎表明质膜面积没有变化， 与许多其他分泌细胞的结果相反。 然而，这些数据是基于整个电池电容测量的 （在已发现RBL细胞不分泌的条件下）， 以及细胞的连续电子显微镜重建（这是一种 不如电容测量精确技术）。 我们建议 进行RBL细胞的透化膜片电容记录，从而 允许电进入整个细胞膜， 维持分泌所必需的细胞质完整性。