GENETIC MANAGEMENT OF ANIMAL COLONIES

描述（改编自申请人摘要）： 逆转录病毒和疱疹B病毒SPF猕猴已经成为研究的主要目标， 国内圈养繁殖计划。 这些SPF菌落对于 开展艾滋病和与艾滋病有关的研究， 照料动物或处理动物组织的人员。 这个目标 该项目旨在开发监测基因多样性， 近亲繁殖、雄性繁殖成功率和SPF遗传细分 比现在更经济、更可靠地繁殖蜂群。 用于高度多态性人类的聚合酶链反应（PCR）引物 将筛选微卫星位点的遗传异质性和孟德尔 隔离在几个SPF和其他生物医学上重要的灵长类物种。 四组三个最有效的基因座，在一个 单一反应，只有满足最低标准的充分性， 基因管理，将开发用于基因管理。 在 每个多态性至少有一个等位基因将被测序以确认片段 大小和重新设计引物，使其与猕猴DNA上的退火位点不匹配。 将使用两个最有效的三位点多重集来估计 用专门设计的计算机进行遗传管理的有用参数 软件 基因多样性、近亲繁殖和遗传细分将是 在第二代后代的最早出生时评估（即， 1997-00年出生的人）的SPF创始人，和遗传管理 基于此的计划，在没有NIH支持的情况下是可持续的， 为每个参与的SPF菌落编写。 这笔赠款的时间安排， 在NIH资助猕猴SPF项目结束后继续六个月， 将允许时间来完成足够的数据分析， 实施自我可持续的遗传管理计划， 连续性

DESCRIPTION (Adapted from the applicant's abstract): The development of retrovirus and herpes B virus SPF macaques has become a primary goal in domestic captive breeding programs. These SPF colonies are important for the conduct of AIDS and AIDS-related research, and in protecting the health personnel who care for animals or handle animal tissue. The goal of this project is to develop methods to monitor parameters such as gene diversity, inbreeding, male reproductive success, and genetic subdivision in SPF breeding colonies more economically and reliably than is presently possible. Polymerase chain reaction (PCR) primers for highly polymorphic human microsatellite loci will be screened for genetic heterogeneity and Mendelian segregation in several SPF and other biomedically important primate species. Four sets of three of the most effective loci that are multiplexible in a single reaction, and that alone meet minimum standards of adequacy for genetic management, will be developed for use in genetic management. At least one allele for each polymorphism will be sequenced to confirm fragment sizes and redesign primers that mismatch the annealing sites on macaque DNA. The two most effective three-locus multiple sets will be used to estimate parameters useful for genetic management with specifically designed computer software. Gene diversity, inbreeding and genetic subdivision will be assessed in the earliest born of the second generation of offspring (i.e., those born during 1997-00) of the SPF founders, and a genetic management plan based on this, sustainable in the absence of NIH support, will be written for each participating SPF colony. The timing of this grant to continue six months beyond the end of NIH funding of the macaque SPF program will permit time to complete sufficient analysis of data for designing and implementing a self-sustainable genetic management program without loss of continuity.