COCAINE SENSITIVE DOPAMINE TRANSPORTER SYNTHESIS

多巴胺转运蛋白是可卡因的关键靶点或“受体” 介导其成瘾特性。 因此了解 控制大脑中多巴胺转运蛋白水平的因素是 对了解药物成瘾的机制至关重要。 这 项目计划开发一种方法来测量合成率， 多巴胺转运蛋白和血清素的降解率 运输机也是。 血清素转运体很重要， 它是抗抑郁药物的靶点或受体。 实质性 已经取得了进展，血清素转运蛋白的周转， 它的半衰期是3.4天。 我们正在 确定多巴胺转运蛋白的相同因素。 资金 NIH /DA 11178 $141，199 08/01/97 - 06/30/00出版物Boja，J.W.， 卡罗尔，F.I.，Vaughan，R.A.，Kopajtic，T.和Kuhar，M.J. Multiple 大鼠体内[125 I]RTI-121和其它可卡因类似物的结合位点 额叶皮层 Synapse 30：9-17，1998. 叮，Y.-美国，莫利纳 体育课，福勒，J.S.，洛根，J.，北达科他州，Kuhar，M. J.和卡罗尔， F.I.地棘蛙素衍生物[18F]NFEP和 [18F] N-甲基-NFEP动力学、尼古丁、效果和毒性。 核 Med. 25：139-148，1999. Kuhar，M.神经递质转运蛋白 不是在Synapse？ 在INABIS '98 -第五届互联网世界上发表 加拿大麦克马斯特大学生物医学科学大会， 十二月七日至十六日。 特邀座谈会。 Kuhar，M.J.，沃恩河，呃， G.，Cerruti角，雷韦河，弗里德角，尼伦堡湾Pickel，V. 多巴胺转运蛋白的显微定位 组织化学 高级药理学 42：168-170，1998. Vaughan，R.A.， 赫夫，R.A.，呃，G.R.和Kuhar，M. J.多巴胺的磷酸化 运输和快速适应可卡因，在戈尔茨坦，南卡罗来纳州， Eisenhofer，G.，和McCarty，R.（编辑）儿茶酚胺桥接碱性 科学与临床医学，学术出版社，圣地亚哥，页。 1042-, 1998. P51 RR 00165 -38 1998年1月1日-1998年12月31日Yerkes区域灵长类动物 研究中心

The dopamine transporter is a key target or "receptor" for cocaine that mediates its addicting properties. Therefore, understanding the factors which control the levels of dopamine transporter in brain is essential for understanding mechanisms of drug addiction. This project plans to develop a method to measure the synthesis rate and degradation rate of the dopamine transporter, and the serotonin transporter as well. The serotonin transporter is important because it is a target or receptor for antidepressant drugs. Substantial progress has been made in that the serotonin transporter turnover has been measured and its half-life is 3.4 days. We are in the process of determining the same factors for the dopamine transporter. FUNDING NIH / DA11178 $141,199 08/01/97 - 06/30/00 PUBLICATIONS Boja, J.W., Carroll, F.I., Vaughan, R.A., Kopajtic, T. and Kuhar, M.J. Multiple binding sites for [125I]RTI-121 and other cocaine analogs in rat frontal cerebral cortex. Synapse 30:9-17, 1998. Ding, Y.-S., Molina, P.E., Fowler, J.S., Logan, J., Volkow, N.D., Kuhar, M.J. and Carroll, F.I. Comparative studies of epibatidine derivatives [18F]NFEP and [18F]N-methyl-NFEP Kinetics, nicotine, effect, and toxicity. Nucl. Med. Biol. 25:139-148, 1999. Kuhar, M. Neurotransmitter transporters are NOT in the Synapse? Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, December 7-16th. Invited Symposium. Kuhar, M.J., Vaughan, R., Uhl, G., Cerruti, C., Revay, R., Freed, C., Nirenburg, M. and Pickel, V. Localization of dopamine transporter protein by microscopic histochemistry. Adv. Pharmacol. 42:168-170, 1998. Vaughan, R.A., Huff, R.A., Uhl, G.R. and Kuhar, M.J. Phosphorylation of dopamine transporters and rapid adaptation to cocaine, in Goldstein, D.S., Eisenhofer, G., and McCarty, R. (Eds.) Catecholamines Bridging Basic Science with Clinical Medicine, Academic Press, San Diego, pp. 1042-, 1998. P51RR00165-38 1/1/98 - 12/31/98 Yerkes Regional Primate Research Center