SPECIFIC PATHOGEN FREE RHESUS MACAQUE BREEDING & RESEARCH PROGRAM: AIDS MODELS

NERPRC SPF计划是富有成效的，并已保持无病原体整整七年。 在最近完成的1996-1997年繁殖季节怀孕率很高，许多年轻的成年女性完成了他们的第二个繁殖季节。 自1993年以来，SPF殖民地的所有增长都是通过内部招募。 繁殖计划成功的一个标志是由第二代SPF动物组成的成年繁殖群体的比例不断增加。 1997年，大约30%的成年雌性育种者将由SPF创始人所生，我们预计随着持续的内部招募和衰老动物的退休，这一数字将在1998年上升到50%。 定期检查所有动物对三种靶逆转录病毒和HBV的抗体是SPF育种群的衍生和维持的关键。 已建立的SPF组现在每半年筛查一次，除非该组中意外的临床疾病需要更频繁的检测。 NERPRC SPF群体具有高生育力，具有良好的母性行为和最少的战斗。 群体生育率平均为70%，新生儿死亡的损失最小。 NERPRC SPF群体表现出良好的母性行为，极少数婴儿因排斥而需要人工抚养。 正如预计的那样，SPF菌落是NERPRC PHS支持的艾滋病研究的重要资源。 殖民地的主要价值是提供研究用动物。 1996年和1997年，大量动物被分配到NERPRC进行研究，预计这一数字将稳定在每年60只。 此外，殖民地中的健康SPF成虫是批准体外研究的血液、骨髓和其他组织标本的重要来源。 1997年，殖民地有388只猕猴。

The NERPRC SPF program is productive and has remained pathogen-free for seven full years. The pregnancy rate in the recently completed 1996-1997 breeding season was high as many young adult females completed their second breeding season. All growth in the SPF colony since 1993 has been through internal recruitment. An indication of the success of the breeding program is the increasing proportion of the adult breeding colony composed of second generation SPF animals. In 1997 approximately 30% of the adult female breeders will have been born to SPF founders, a figure that we project will rise to 50% in 1998 with continued internal recruitment and retirement of senescent animals. Regular examination of all animals for antibody to the three target retroviruses and HBV is key to derivation and maintenance of SPF breeding groups. Established SPF groups are now screened semiannually unless more frequent testing is warranted by unexpected clinical illness in the group. The NERPRC SPF colony experiences high fertility with good maternal behavior and minimal fighting. Colony fertility averages 70%, with minimal losses from neonatal death. The NERPRC SPF colony exhibits excellent maternal behavior, with very small numbers of infants requiring hand-rearing because of rejection. As projected, the SPF colony is a vital resource for PHS-supported AIDS research at NERPRC. The main value of the colony is to provide animals for research. Large numbers of animals have been assigned to studies at NERPRC in 1996 and 1997, a figure that is projected to stabilize at 60 per year. In addition, healthy SPF adults in the colony are an important source for blood, bone marrow and other tissue specimens for approved in vitro studies. In 1997, the colony consisted of 388 macaques.