SPECIFIC PATHOGEN FREE RHESUS MACAQUE BREEDING & RESEARCH PROGRAM: AIDS MODELS

无特定病原体恒河猴育种

• 批准号: 6277813
• 负责人: PRABHAT K SEHGAL
• 金额: $ 8.77万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277813
• 关键词: AIDS; Mammalia; Primates; immunology; infection; inflammation; lymphatic system; reproductive system; virus

The NERPRC SPF program is productive and has remained pathogen-free for seven full years. The pregnancy rate in the recently completed 1996-1997 breeding season was high as many young adult females completed their second breeding season. All growth in the SPF colony since 1993 has been through internal recruitment. An indication of the success of the breeding program is the increasing proportion of the adult breeding colony composed of second generation SPF animals. In 1997 approximately 30% of the adult female breeders will have been born to SPF founders, a figure that we project will rise to 50% in 1998 with continued internal recruitment and retirement of senescent animals. Regular examination of all animals for antibody to the three target retroviruses and HBV is key to derivation and maintenance of SPF breeding groups. Established SPF groups are now screened semiannually unless more frequent testing is warranted by unexpected clinical illness in the group. The NERPRC SPF colony experiences high fertility with good maternal behavior and minimal fighting. Colony fertility averages 70%, with minimal losses from neonatal death. The NERPRC SPF colony exhibits excellent maternal behavior, with very small numbers of infants requiring hand-rearing because of rejection. As projected, the SPF colony is a vital resource for PHS-supported AIDS research at NERPRC. The main value of the colony is to provide animals for research. Large numbers of animals have been assigned to studies at NERPRC in 1996 and 1997, a figure that is projected to stabilize at 60 per year. In addition, healthy SPF adults in the colony are an important source for blood, bone marrow and other tissue specimens for approved in vitro studies. In 1997, the colony consisted of 388 macaques.

NERPRC的SPF计划是富有成效的，并一直保持着 整整七年没有病原体。中国的怀孕率 刚刚结束的1996-1997繁殖季 成年雌性完成了第二个繁殖季节。所有增长都在 SPF殖民地自1993年以来一直通过内部招募。一个 育种计划取得成功的标志是 由第二代组成的成虫繁殖群体的比例 SPF动物。1997年，大约30%的成年雌性繁殖者 将由SPF创始人所生，我们预计这一数字将 在继续内部招聘和退休的情况下，1998年上升到50% 衰老的动物。定期检查所有动物的抗体 对三种靶标逆转录病毒和乙肝病毒的关键是衍生和 SPF繁育群的维护。已建立的SPF组现在 每半年进行一次筛查，除非 小组中有意想不到的临床疾病。NERPRC SPF殖民地 经历高生育率、良好的母性行为和最低限度的 打架。群体生育率平均为70%，损失最小的是 新生儿死亡。NERPRC SPF菌落展示了出色的母体 行为，需要人工抚养的婴儿数量很少 因为被拒绝了。正如预测的那样，SPF菌落是一个至关重要的 NERPRC的小灵通支持的艾滋病研究资源。的主要价值 这个殖民地将为研究提供动物。大量的 1996年和1997年，动物被分配到NERPRC进行研究， 这一数字预计将稳定在每年60人。此外, 群体中健康的SPF成虫是血液的重要来源， 经批准的体外研究用骨髓和其他组织标本。 1997年，这个群体由388只猕猴组成。