GROWTH OF NON STARTER LACTIC ACID BACTERIA IN REDUCED FAT CHEESE

低脂奶酪中非发酵剂乳酸菌的生长

• 批准号: 6120999
• 负责人: JAMES L STEELE
• 金额: $ 0.04万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120999
• 关键词: biological products; biomaterials; biomedical resource; nuclear magnetic resonance spectroscopy; proteins

EK18 is a mutant of trp repressor and is cal a "super-repressor" since it represses the transcription of genes responsible for typtophan synthesis at a lower level of tryptophan molecules in the cell. Its molecular weight is about 12.5 kDaltons per monomer and it exists primarily as a dimer. However, when the salt concentration is lowered, or the protein concentration is increased, oligomerization occurs between dimers. The protein-protein interaction seems to play a role in the mechanism of repression. In addition to the corepressor concentration, this oligomerization may act to fine tune the gene regulation. Several studies have been done using fluorescence spectroscopy to investigate the oligomerization, but no structural studies have been done to date. NMR studies of this oligomerization will tell how the two dimers interact (i.e., whether the tetramerization is specific or nonspecific, or whether is it symmetric or unsymmetric).

EK18是trp抑制因子的突变体，被称为“超级抑制因子”。 因为它抑制了负责基因的转录 在较低水平的色氨酸分子中合成色氨酸 手机。它的分子量约为12.5kDalton/单体，它 主要以二聚体的形式存在。然而，当盐浓度为 降低或提高蛋白质浓度，齐聚 发生在二聚体之间。蛋白质之间的相互作用似乎在起作用。 在压制机制中扮演的角色。除了辅抑制子 浓度，这种寡聚作用可能会微调基因 监管。已经进行了几项使用荧光技术的研究 光谱学研究齐聚反应，但没有结构 到目前为止，已经进行了一些研究。这种齐聚反应的核磁共振研究 会告诉我们这两个二聚体是如何相互作用的(即 四聚化是特定的还是非特定的，或者它是否是对称的 或不对称)。