ONTOGENY OF INTRAGONADAL NEURONAL SYSTEM IN PRIMATE OVARY

我们之前已经描述了儿茶酚胺能 表达低亲和力神经生长因子受体的神经元 在恒河猴的卵巢里。我们现在正在评估产后 这些NGFR阳性细胞的个体发育以及它们的 从新生儿到衰老的卵巢中儿茶酚胺能功能 动物。神经生长因子受体的免疫细胞化学(ICC)检测显示 其中最多的神经元出现在新生儿的卵巢中， 随着出生后生命中的数量逐渐减少，所以 到了20岁，这些只是偶然出现的神经元。国际刑事法院 酪氨酸羟化酶(TH)，揭示了神经元的能力 在新生儿(2个月)的卵巢中产生儿茶酚胺的水平最低，然后 在青少年时期(8个月)和在 青春期(3.5岁)发育。我们的最新数据，尽管 初步研究表明，TH阳性神经元的数量下降 逐渐地，直到大约12岁。随后， 这些神经元的数量在17岁时显著减少，成为 在20-27岁的猴子中几乎不存在。我们还有 启动实验以确定先天性心脏病的起源和产前个体发育 卵巢神经元。我们已经在猴子体内确定了NGFR神经元 最早100天的胎儿的卵巢。不幸的是，我们已经 难以获得更年轻的胎儿。然而，我们最近 成年猪神经生长因子受体/TH阳性神经元的发现 卵巢，现在正在按顺序收集不同年龄的猪胚胎 继续进行产前个体发育研究。到目前为止，我们已经获得 有证据表明神经元(其中一些是TH阳性的) 从发育中的神经管中迁移出来，并且已经在 胚胎第23天，生殖器脊和未分化的性腺。早些时候 目前正在收集年龄。

We have previously described the presence of catecholaminergic neurons expressing low affinity nerve growth factor receptors (NGFR) in the rhesus monkey ovary. We are now assessing the postnatal ontogeny of these NGFR-positive cells, as well as their catecholaminergic capabilities in ovaries from neonatal to senescent animals. Immunocytochemistry (ICC) for NGFR revealed that the greatest number of these neurons were present in neonatal ovaries, with the numbers gradually declining throughout postnatal life, so that these were only occasional neurons by 20 years of age. ICC for tyrosine hydroxylase (TH), revealed that the capability of the neurons to produce catecholamines was lowest in neonatal (2 mo) ovaries, then increased dramatically during juvenile (8 mo) and again during peripubertal (3.5 yr) development. Our most recent data, although preliminary, suggests that the number of TH-positive neurons decline gradually until approximately 12 years of age. Subsequently, the number of these neurons declined markedly by 17 years of age, becoming virtually absent in monkeys reaching 20-27 years of age. We have also initiated experiments to determine the origin and prenatal ontogeny of ovarian neurons. We have positively identified NGFR-neurons in monkey ovaries from fetuses as early as 100 days. Unfortunately, we have had difficulty in obtaining younger fetuses. We have, however, recently discovered the presence of NGFR/TH-positive neurons in adult pig ovaries, and are now collecting pig fetuses at various ages in order to continue the prenatal ontogeny study. To date, we have obtained evidence that the neurons (some of which are TH-positive) are migrating from the developing neural tube and are already within the genital ridge and the undifferentiated gonad by fetal day 23. Earlier ages are currently being collected.