MICROGRAPH WKSHP: ANNOTATION & ANALYSIS OF EM MONTAGE CROSS PLATFORM APPLICATION

显微照片 WKSHP：注释

• 批准号: 6117258
• 负责人: John Graham White
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117258
• 关键词: bioimaging /biomedical imaging; biomedical resource; computers; microscopy; technology /technique

Summary Using the cross-platform programming language, Java, an application is being developed which will allow the user to create large montages of EM images. In this way, large areas of the sample can be visualized at high resolution using the electron microscope. Subsequently, the individual images of the montage can be loaded into the application and reassembled into a single large image. Images can be scaled up or down in size, and scrolled to make viewing easier. Individual areas as well as the entire assembled montage can be saved to and recalled from the computer's hard disk. Annotation tools, similar to those available for the IMR's "4D Viewer" application, allow the user to create color overlays with circles, squares, freehand and straight lines, text, and arrows which can elucidate specific features of interest in the image montage. Additionally, each overlay object can be further described with searchable hidden text boxes. These overlays can be saved to and recalled from the computer's hard disk. Future Directions: Once tools are available to allow Java to use compression algorithms, we hope to add the ability to compress the large image montages and save them to the disk as compressed files. In this way, extremely large montages could be handled more easily. We also plan to continue adding features as testing deems them necessary.

摘要使用跨平台编程语言Java和 正在开发的应用程序将允许用户创建 大量的EM图像剪辑。这样，大面积的样本 可以用电子显微镜以高分辨率可视化。 随后，可以将蒙太奇的各个图像加载到 应用程序并重新组合成单个大图像。图像可以 可以放大或缩小大小，并滚动以使查看更容易。 可以保存单个区域以及整个组装的剪辑 到计算机硬盘并从计算机硬盘中召回。注释工具、 与IMR的“4D查看器”应用程序类似， 允许用户创建带有圆形、正方形、 手绘和直线、文本和箭头，可以阐明 图像剪辑中感兴趣的特定特征。另外， 每个覆盖对象都可以用可搜索的隐藏来进一步描述 文本框。可以将这些覆盖图保存到 计算机的硬盘。未来方向：一旦有工具可用来 允许Java使用压缩算法，我们希望添加这个能力 压缩大图像剪辑并将其保存到磁盘 压缩文件。通过这种方式，极大的蒙太奇可以 更容易处理。我们还计划继续添加功能，如 测试认为它们是必要的。