SYNCHROTRON CRYSTALLOGRAPHY OF GTPASES & GUANYLATE KINASES

GTP酶的同步加速器晶体学

• 批准号: 6120419
• 负责人: XINHUA JI
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120419
• 关键词: biological products; biomedical resource; enzymes; spectrometry

The following structures have been solved or datasets collected: apo-Guanylate Kinase: Hg MAD data at 2.0 A, four wavelengths. Structure has been solved by MAD phasing and the refinement at 1.8 A is in progress. ERA GTPase: SeMet MAD data at 3.2 A, four wavelengths. We were not able to solve for the 16 Se sites due to the low resolution and low signal/noise ratio. ERA GTPase: native data at 2.6 A. apo-Guanylate Kinase: native data at 1.8 A. ERA GTPase: Hg MAD data at 2.6 A, four wavelengths. MAD phases have a FOM of 0.60 before solvent flattening. Tracing is in progress.

已经解决了以下结构或收集了数据集： Apo-Guanyate Kinase：HG-MAD数据为2.0A，四个波长。 结构通过MAD相移和1.8A的精化得到了解决 正在进行中。Era GTP酶：SMET MAD数据在3.2 A，4 波长。我们无法解决16个Se地点的问题，因为 分辨率低，信噪比低。Era GTP酶：原生数据 2.6A.apo-Ganyate Kinase：1.8A.ERA GTPase：HG MAD数据，2.6A，四个波长。MAD相的FOM为0.60 在溶剂压平之前。正在进行跟踪。