SYNCHROTRON CRYSTALLOG OF 7,8 DIHYDRO 6 HYDROXYMETHYLPTERIN PYROPHOSPHOKINASE

7，8-二氢-6-羟甲基蝶呤-焦磷酸激酶（HPPK）具有 从E.杆菌 它是叶酸中的第一种酶 合成. 不像哺乳动物细胞， 在饮食中，大多数微生物必须重新合成叶酸，因为 它们缺乏运输途径。 因此，HPPK非常重要。 作为选择性抗菌化疗的潜在靶点。 数据 对于天然蛋白质，已经以1.6E分辨率收集（空间 P21组，a=36.3 E，B=51.9 E，c=41.2 E，t =110.3，Rsym=4.4%。 其他 收集的数据：HPPK / MgATP复合物：1.6处的单波长数据 A. HPPK / HP复合物：2.1 A处的单波长数据。

7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK) has been expressed from E. coli. It is the first enzyme in folate synthesis. Unlike mammalian cells that take up folate cofactors from the diet, most microorganisms must synthesize folates de novo, since they lack the transport pathway. For this reason, HPPK is important as a potential target for selective antimicrobial chemotherapy. Data for native protein have been collected at 1.6 E resolution (space group P21, a=36.3 E, b=51.9 E, c=41.2 E, =110.3 , Rsym=4.4%). Other data collected: HPPK / MgATP complex: single wavelength data at 1.6 A. HPPK / HP complex: single wavelength data at 2.1 A.