RAT BASOPHILIC LEUKEMIA (RBL) CELL ACTIVATION

细胞激活途径对过敏和哮喘的重要作用 正在通过转染RBL(大鼠嗜碱性白血病)细胞进行检测 与甲酰肽受体结合。这允许比较信号 由不同机制启动的转导通路可导致 相似的细胞反应。主要途径涉及G蛋白 甲酰肽和受体激酶介导的信号转导 免疫球蛋白E受体交联化所引发的相关信号 抗原。用流式细胞仪对转基因细胞进行了鉴定 信号通路和运动反应的几个方面 已经评估过了。这些包括肌动蛋白聚合，磷酸酪氨酸 通过流式细胞术检测激活剂的激活和钙离子的反应。二 论文已经发表(Hall等人，J.Leuk)。比奥尔。1997年；Lee et 艾尔BBRC，1997)

The pathways of cell activation important to allergy and asthma are being examined by transfecting RBL (rat basophilic leukemia) cells with formyl peptide receptors. This permits the comparison of signal transduction pathways initiated by distinct mechanisms but leading to similar cell response. The primary pathways involve G protein mediated signals initiated by formyl peptide and receptor kinase associated signals initiated by crosslinking of the IgE receptor by antigen. Transfected cells are being characterized by flow cytometry and several aspects of the signalling pathway and motile response have been evaluated. These include actin polymerization, phosphotyrosine kinase activation, and calcium response, all by flow cytometry. Two paper have been published (Hall et al., J. Leuk. Biol. 1997; Lee et al. BBRC, 1997)