RAT BASOPHILIC LEUKEMIA (RBL) CELL ACTIVATION

大鼠嗜碱性白血病 (RBL) 细胞激活

• 批准号: 6298056
• 负责人: LARRY A. SKLAR
• 金额: $ 0.36万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6298056
• 关键词: biomedical resource; hematology; immunology; infection; inflammation; technology /technique

The pathways of cell activation important to allergy and asthma are being examined by transfecting RBL (rat basophilic leukemia) cells with formyl peptide receptors. This permits the comparison of signal transduction pathways initiated by distinct mechanisms but leading to similar cell response. The primary pathways involve G protein mediated signals initiated by formyl peptide and receptor kinase associated signals initiated by crosslinking of the IgE receptor by antigen. Transfected cells are being characterized by flow cytometry and several aspects of the signalling pathway and motile response have been evaluated. These include actin polymerization, phosphotyrosine kinase activation, and calcium response, all by flow cytometry. Two paper have been published (Hall et al., J. Leuk. Biol. 1997; Lee et al. BBRC, 1997)

细胞活化的途径对过敏和哮喘很重要 正在通过分离RBL（大鼠嗜碱性白血病）细胞进行检查 与甲酰肽受体结合。 这允许信号的比较 由不同的机制启动的转导途径， 类似的细胞反应。 主要途径涉及G蛋白 由甲酰肽和受体激酶启动的介导信号 由IgE受体的交联引发的相关信号， 抗原的 通过流式细胞术对转染细胞进行表征 信号通路和运动反应的几个方面 被评估。 这些包括肌动蛋白聚合、磷酸酪氨酸 激酶活化和钙应答，全部通过流式细胞术。 两 论文已经发表（Hall等人，勒克。 1997; Lee等人 al. BBRC，1997年）