RAT BASOPHILIC LEUKEMIA (RBL) CELL ACTIVATION

大鼠嗜碱性白血病 (RBL) 细胞激活

• 批准号: 6280958
• 负责人: LARRY A. SKLAR
• 金额: $ 0.36万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280958
• 关键词: biomedical resource; hematology; immunology; infection; inflammation; technology /technique

The pathways of cell activation important to allergy and asthma are being examined by transfecting RBL (rat basophilic leukemia) cells with formyl peptide receptors. This permits the comparison of signal transduction pathways initiated by distinct mechanisms but leading to similar cell response. The primary pathways involve G protein mediated signals initiated by formyl peptide and receptor kinase associated signals initiated by crosslinking of the IgE receptor by antigen. Transfected cells are being characterized by flow cytometry and several aspects of the signalling pathway and motile response have been evaluated. These include actin polymerization, phosphotyrosine kinase activation, and calcium response, all by flow cytometry. Two paper have been published (Hall et al., J. Leuk. Biol. 1997; Lee et al. BBRC, 1997)

对过敏和哮喘很重要的细胞激活途径 正在通过转染 RBL（大鼠嗜碱性白血病）细胞进行检查 与甲酰肽受体。 这允许比较信号 由不同机制启动但导致的转导途径 类似的细胞反应。 主要途径涉及 G 蛋白 由甲酰肽和受体激酶引发的介导信号 IgE 受体交联引发的相关信号 抗原。 通过流式细胞术表征转染细胞 信号通路和运动反应的几个方面 被评估。 这些包括肌动蛋白聚合、磷酸酪氨酸 激酶激活和钙反应，全部通过流式细胞术进行。 二 论文已发表（Hall 等人，J. Leuk. Biol. 1997；Lee 等人） 等人。英国广播公司，1997）