Core--Transgenic mouse/ES cell

核心--转基因小鼠/ES细胞

• 批准号: 6395761
• 负责人: MARK A MAGNUSON
• 金额: --
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6395761
• 关键词: biomedical facility; cryopreservation; embryonic stem cell; gene targeting; genetically modified animals; laboratory mouse; microinjections; neoplasm /cancer; oncology; tissue mosaicism

The transgenic Mouse/ES Cell Shared Resource consists of both the Microinjection and ES Cell Laboratories. Together, these two components provide seven different services that facilitate the generation, maintenance, or storage of transgenic and gene knock- out mice. Over the past three and a half years this resource has served 35 different Cancer Center investigators (of a total of 50 different investigators). The Microinjection Laboratory has generated over 273 transgenic founder mice (made via DNA microinjections) and 508 chimeric mice (made via ES cell microinjections). The ES Cell laboratory, which began operation in July, 1995, has assisted in the generation of at least nine different gene-targeted mice. Four other new services have been developed and are now provided to investigators. These include rederivation of established lines, assisted reproduction, embryo cyropreservation, and rental of a microinjection apparatus for investigators who want to perform their own microinjection experiments. This resource, which has been successful in meeting requests for its services, is expected to continue to provide both existing and new services as the demand for germline-altered mice in biomedical investigation continues to grow.

转基因小鼠/胚胎干细胞共享资源由显微注射实验室和胚胎干细胞实验室组成。这两个组成部分总共提供了七种不同的服务，以促进转基因和基因敲除小鼠的生成、维护或存储。在过去的三年半里，这一资源为35名不同的癌症中心调查人员(总共50名不同的调查人员)提供了服务。显微注射实验室已经产生了273只转基因方正小鼠(通过DNA微注射获得)和508只嵌合小鼠(通过ES细胞微注射获得)。胚胎干细胞实验室于1995年7月开始运作，已经帮助产生了至少9种不同的基因靶标小鼠。还开发了另外四项新服务，现已提供给调查人员。这些措施包括重新衍生已建立的品系、辅助生殖、胚胎冷冻保存，以及为想要进行自己的微注射实验的研究人员租用微注射设备。这一资源已经成功地满足了对其服务的要求，随着生物医学研究中对生殖系改变小鼠的需求继续增长，预计将继续提供现有和新的服务。