INVESTIGATION OF BETA HAIRPIN FORMATION IN WATER

我们对短肽的二级结构感兴趣 水溶液。我们小组之前的研究表明， 一系列含16-MERS的D-Pro采用单体发夹 水溶液中的构象，而它们的全L立体异构体 不。我们计划探讨发夹的基本要求 在水中形成队形。第一个目标将是确定 一个稳定的发夹所需的最少残留物数量。这个 第二个目标将是进行结构-活动研究，目的是 了解特定残基和残基间的相互作用 有助于b型发夹的稳定性。旨在包含以下成分的多肽 还将分析多个发夹。我们小组之前已经 识别？-在有机和水介质中形成发夹 核磁共振技术。COSY和TOCSY实验促进峰值 作业，而NOESY和/或ROESY实验提供信息 关于三维解的结构。其他内容 合成肽的实验表征技术 将包括圆二色谱和红外光谱研究。

We are interested in the secondary structure of short peptides in aqueous solutions. Previous studies within our group have shown that a series of D-Pro containing 16-mers adopt monomeric ?-hairpin conformations in aqueous solution, while their all-L stereoisomers do not. We plan to probe the fundamental requirements for ?-hairpin formation in water. The first objective will be to determine the minimum number of residues necessary for a stable ?-hairpin. The second goal will be to perform structure-activity studies with the aim of learning how specific residues and interresidue interactions contribute to b-hairpin stability. Peptides designed to contain multiple hairpins will also be analyzed. Our group has previously identified ?-hairpin formation in both organic and aqueous media using NMR techniques. COSY and TOCSY experiments facilitate peak assignments, while NOESY and/or ROESY experiments provide information regarding the three-dimensional solution structure. Additional experimental techniques for characterization of synthesized peptides will include circular dichroism and IR studies.