NMR STUDIES OF C2 DOMAINS OF SYNAPTOTAGMIN I

突触结合蛋白 I C2 结构域的 NMR 研究

基本信息

  • 批准号:
    6309103
  • 负责人:
  • 金额:
    $ 0.75万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-04-15 至 2005-02-28
  • 项目状态:
    已结题

项目摘要

The synaptotagmin is involved in the Ca2+-induced neurotrasmitter release. The synaptotagmin has roughly 4 domains: intravesicular domain, transmembrane domain, and 2 cytosolic domains. Two cytosolic domains are named C2A and C2B. The first domain is the one to study by NMR. C2A domain is known to be a Ca2+ sensor in the membrane exocytosis. According to the Ca2+ binding assay in the presence of phospholipids, the Hill coefficient is larger than 3, which in turn means C2A should bind at least 3 Ca2+ ions. So far, the x-ray structure found one Ca2+ binding site, and NMR data suggested the second site. Very recently, the x-ray structure of the PLC-?1 which has a C2 domain proposed the third binding site based on the homology of the two structures. The failure in finding the third binding site is presumed to result from the absence of the lipids since the intrinsic affinity of C2A domain to Ca2+ is low. In the current NMR study of this C2A domain, a short version of the phospholipid has been used. NMR data show changes in the chemical shifts with the presence of Ca2+ or lipid or both, which strongly suggests a existence of a ternary complex. The goal of this study is to solve the structure of the ternary complex: C2A-Ca2+-lipid.
突触蛋白参与Ca2+诱导的神经递质

项目成果

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JOHN LUTE MARKLEY其他文献

JOHN LUTE MARKLEY的其他文献

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{{ truncateString('JOHN LUTE MARKLEY', 18)}}的其他基金

Biogenesis of human mitochondrial iron-sulfur proteins
人类线粒体铁硫蛋白的生物合成
  • 批准号:
    10001537
  • 财政年份:
    2019
  • 资助金额:
    $ 0.75万
  • 项目类别:
The BMRB as an evolving resource for biomolecular structure-function research
BMRB 作为生物分子结构功能研究的不断发展的资源
  • 批准号:
    9462715
  • 财政年份:
    2014
  • 资助金额:
    $ 0.75万
  • 项目类别:
The BMRB as an evolving resource for biomolecular structure-function research
BMRB 作为生物分子结构功能研究的不断发展的资源
  • 批准号:
    8615052
  • 财政年份:
    2014
  • 资助金额:
    $ 0.75万
  • 项目类别:
The BMRB as an evolving resource for biomolecular structure-function research
BMRB 作为生物分子结构功能研究的不断发展的资源
  • 批准号:
    9253407
  • 财政年份:
    2014
  • 资助金额:
    $ 0.75万
  • 项目类别:
The BMRB as an evolving resource for biomolecular structure-function research
BMRB 作为生物分子结构功能研究的不断发展的资源
  • 批准号:
    8852654
  • 财政年份:
    2014
  • 资助金额:
    $ 0.75万
  • 项目类别:
METABOLITE CHANGES IN E COLI STRAINS EVOLVED TO BE RADIATION RESISTANT
大肠杆菌菌株的代谢物变化进化为抗辐射性
  • 批准号:
    8361207
  • 财政年份:
    2011
  • 资助金额:
    $ 0.75万
  • 项目类别:
METHANOCALDOCOCCUS JANNASCHII COBY (MJ1117)
甲烷热球菌 JANNASCHII COBY (MJ1117)
  • 批准号:
    8361210
  • 财政年份:
    2011
  • 资助金额:
    $ 0.75万
  • 项目类别:
STRUCTURAL ANALYSIS FOR PROTEINS FROM CESG
CESG 蛋白质的结构分析
  • 批准号:
    8361246
  • 财政年份:
    2011
  • 资助金额:
    $ 0.75万
  • 项目类别:
TIME IDLE & OUT OF SERVICE
空闲时间
  • 批准号:
    8361151
  • 财政年份:
    2011
  • 资助金额:
    $ 0.75万
  • 项目类别:
RELATIONSHIPS BETWEEN REDOX POTENTIAL, HYPERFINE SHIFTS, AND THE PKA(S)
氧化还原电位、超精细位移和 PKA 之间的关系
  • 批准号:
    8361161
  • 财政年份:
    2011
  • 资助金额:
    $ 0.75万
  • 项目类别:

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