Selection for antibiotic resistance in complex microbial communities

复杂微生物群落中抗生素耐药性的选择

• 批准号: 1641426
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1641426
• 关键词: Selection; antibiotic; resistance; complex; microbial

It is becoming increasingly clear that the natural environment plays an important role in the evolution and dissemination of antibiotic resistance. This studentship will determine the mechanistic basis and significance of gene flow that underlies the abundance of antimicrobial resistance genes in bacteria inhabiting polluted natural environments such as downstream of waste water treatment plants (WWTPs). It is unclear whether increased prevalence of antibiotic resistant organisms associated with WWTPs is due to constant emissions of resistant organisms or whether bacteria such as multi-drug resistant E. coli persist and/or acquire resistance genes by horizontal gene transfer (HGT) in aquatic sediments under selection by environmental pollutants including antibiotic residues. This project will study selection for resistance in complex communities utilising mesocosm studies interrogated using culture and culture independent methods. The latter will include real-time PCR for enumeration of key marker genes and next generation sequencing (NGS) to determine the effects of single antibiotics, mixtures of different classes of antibiotic and field samples of polluted effluents on relative abundance of all known resistance genes. This knowledge will be used to support environmental policy development and environmental risk management.

越来越清楚的是，自然环境在抗生素耐药性的进化和传播中发挥着重要作用。该奖学金将确定基因流的机制基础和重要性，该基因流是居住在污染自然环境（例如废水处理厂（WWTP）下游）的细菌中大量抗菌素耐药性基因的基础。目前尚不清楚与污水处理厂相关的抗生素抗性生物的流行率增加是否是由于抗性生物的不断排放，或者是由于多重耐药大肠杆菌等细菌在包括抗生素残留在内的环境污染物的选择下在水生沉积物中持续存在和/或通过水平基因转移（HGT）获得抗性基因。该项目将利用文化和独立于文化的方法进行的中生态研究来研究复杂群落中抗性的选择。后者将包括用于计数关键标记基因的实时 PCR 和下一代测序 (NGS)，以确定单一抗生素、不同类别抗生素的混合物以及污染废水的现场样品对所有已知抗性基因相对丰度的影响。这些知识将用于支持环境政策的制定和环境风险管理。

项目成果

The 'SELection End points in Communities of bacTeria' (SELECT) Method: A Novel Experimental Assay to Facilitate Risk Assessment of Selection for Antimicrobial Resistance in the Environment.

• DOI: 10.1289/ehp6635
• 发表时间: 2020-10
• 期刊: Environmental health perspectives
• 影响因子: 10.4
• 作者: Murray AK;Stanton IC;Wright J;Zhang L;Snape J;Gaze WH
• 通讯作者: Gaze WH

Selection for antibiotic resistance in complex microbial communities

复杂微生物群落中抗生素耐药性的选择

• 发表时间: 2020
• 作者: Stanton I.

Dissipation of the antibiotic sulfamethoxazole in a soil amended with anaerobically digested cattle manure.

• DOI: 10.1016/j.jhazmat.2019.120769
• 发表时间: 2019-10
• 期刊: Journal of hazardous materials
• 影响因子: 13.6
• 作者: J. Rauseo;J. Rauseo;A. Caracciolo;N. Ademollo;Martina Cardoni;M. D. Lenola;William H. Gaze;I. Stanton;P. Grenni;T. Pescatore;T. Pescatore;F. Spataro;L. Patrolecco

Sulfamethoxazole persistence in a river water ecosystem and its effects on the natural microbial community and Lemna minor plant

• DOI: 10.1016/j.microc.2019.103999
• 发表时间: 2019-09-01
• 期刊: MICROCHEMICAL JOURNAL
• 影响因子: 4.8
• 作者: Grenni, Paola;Patrolecco, Luisa;Caracciolo, Anna Barra
• 通讯作者: Caracciolo, Anna Barra

Antimicrobial Resistance: Investigating the Environmental Dimension.

抗菌素耐药性：调查环境因素。

• 发表时间: 2017
• 作者: Gaze W.H.