HIGH RESOLUTION PROTON MR SPECTROSCOPY OF HIV INFECTED PATIENTS

这项研究的目的是开发新的方法学来 测量老年人的肌肉新陈代谢和血流量，两者都有 血流量正常和减少(外周血管疾病)。进展 今年正在测试一种使用部分袖带缺血的方法 在从次极限运动中恢复的过程中。使用MRS，我们测量了PCR值 用血压袖带(放在大腿上)恢复 充气至0、50、60、70和90毫米汞柱。也进行了类似的测量 应用双功多普勒测定局部动脉血流的相对效应 袖带压力对肌肉血液流动的影响。氧的近红外光谱测量 在MRS和多普勒测量期间收集饱和度。 初步结果表明，正常受试者在聚合酶链式反应中没有损伤。 采收率高达25%。拥有更大的流量 减少，流量和聚合酶链式反应的恢复都受到损害。意义重大 近红外光谱恢复率与峰值多普勒血流呈正相关 轻度PVD患者缺血后。接受的级别 再现性表明，更多的数据将允许 血流量和血流量相对贡献率的测定 线粒体容量在决定老年人肌肉代谢中的作用 健康的和患病的受试者。

The purpose of this study was to develop new methodologies to measure muscle metabolism and blood flow in older humans, both with normal and reduced blood flow (peripheral vascular disease). Progress in this year was testing of a method using partial cuff ischemia during recovery from submaximal exercise. With MRS, we measure PCr recovery with a blood pressure cuff (positioned on the upper leg) inflated to 0, 50, 60, 70, and 90 mmHg. Similar measurements are made using duplex Doppler to determine the relative effect of the partial cuff pressures on muscle blood flow. NIRS measurements of oxygen saturation were collected during both MRS and Doppler measurements. Preliminary results suggest normal subjects have no impairment in PCr recovery with flow reductions up to 25%. With greater flow reductions, both flow and PCr recovery are impaired. Significant correlations were found between NIRS recovery and peak Doppler flow after ischemia in patients with mild PVD. Acceptab le levels of reproducibility suggest additional data will allow mathematical determination of relative contributions of blood flow and mitochondrial capacity in determining muscle metabolism in elderly healthy and diseased subjects.