HIGH RESOLUTION PROTON MR SPECTROSCOPY OF HIV INFECTED PATIENTS

HIV 感染患者的高分辨率质子磁共振波谱检查

• 批准号: 6495478
• 负责人: KIM M CECIL
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6495478
• 关键词: aging; biomedical resource; enzymes; female; human subject; musculoskeletal system; technology /technique

The purpose of this study was to develop new methodologies to measure muscle metabolism and blood flow in older humans, both with normal and reduced blood flow (peripheral vascular disease). Progress in this year was testing of a method using partial cuff ischemia during recovery from submaximal exercise. With MRS, we measure PCr recovery with a blood pressure cuff (positioned on the upper leg) inflated to 0, 50, 60, 70, and 90 mmHg. Similar measurements are made using duplex Doppler to determine the relative effect of the partial cuff pressures on muscle blood flow. NIRS measurements of oxygen saturation were collected during both MRS and Doppler measurements. Preliminary results suggest normal subjects have no impairment in PCr recovery with flow reductions up to 25%. With greater flow reductions, both flow and PCr recovery are impaired. Significant correlations were found between NIRS recovery and peak Doppler flow after ischemia in patients with mild PVD. Acceptab le levels of reproducibility suggest additional data will allow mathematical determination of relative contributions of blood flow and mitochondrial capacity in determining muscle metabolism in elderly healthy and diseased subjects.

这项研究的目的是开发新的方法， 测量老年人的肌肉代谢和血流量， 血流量正常和减少（外周血管疾病）。 进展 在这一年， 在从次极量运动中恢复时。 通过MRS，我们测量PCr 恢复时使用血压袖带（位于大腿上） 充盈至0、50、60、70和90 mmHg。 进行了类似的测量 使用双工多普勒来确定局部的相对影响， 袖带压力对肌肉血流的影响。 NIRS测量氧气 在MRS和多普勒测量期间收集饱和度。 初步结果提示正常人PCr无损害 流量减少高达25%的回收率。 流量更大 减少，流量和PCr恢复都受到损害。 显著 NIRS恢复率与峰值多普勒血流之间存在相关性 缺血后轻度PVD患者。 可接受水平 重复性表明，额外的数据将允许数学 确定血流的相对贡献， 线粒体在老年人肌肉代谢中的作用 健康和患病的受试者。