NTP DATABASE SUMMARIZATION AND EVALUATION

NTP数据库总结与评估

• 批准号: 6432303
• 负责人: JOSEPH K HASEMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432303
• 关键词: cancer information system; chemical carcinogen; chemical carcinogenesis; chemical information system; decision making; health science research analysis /evaluation; human data

The goal of this project is to summarize, evaluate, and interpret data contained in the NTP rodent carcinogenicity database and other large databases. As the NTP carcinogenicity database becomes increasingly accessible to the general scientific community, there is increased opportunity for inadvertent misuse of the database by those who may not have a complete understanding of the complex information it contains. We demonstrated that a previously published evaluation of the NTP database exaggerated the frequencies of statistically significant decreasing tumor trends, The factors contributing to these errors included failure to adjust for survival differences, evaluating improper tumor combinations, failure to achieve the reported (p<0.01) level of significance, double counting a chemical's site-specific decreased tumor incidence, and using scientifically inadequate studies. In another project, we evaluated the large database of results from the Frog Embryo Teratogenesis Assay - Xenopus (FETAX), as part of an ICCVAM (Interagency Coordinating Committee on the Validation of Alternative Methods) initiative, We found that in some cases there was significant inter-laboratory variability in the reported malformation rates that appeared to be related to diagnostic differences among investigators, Based on these results, specific recommendations were made as to how the FETAX protocols could be improved to reduce this variability and facilitate any future validation of this assay.

本项目的目标是总结、评价和解释NTP啮齿动物致癌性数据库和其他大型数据库中包含的数据。随着NTP致癌性数据库越来越多地被一般科学界所访问，可能不完全了解其中所包含的复杂信息的人无意中滥用数据库的机会也越来越多。 我们证明，先前发表的NTP数据库评估夸大了统计学显著性肿瘤下降趋势的频率，导致这些错误的因素包括未能调整生存差异，评估不当的肿瘤组合，未能达到报告的（p<0.01）显著性水平，重复计算化学物质的部位特异性降低肿瘤发生率，并使用科学上不充分的研究。 在另一个项目中，作为ICCVAM的一部分，我们评估了蛙胚胎致畸试验-非洲爪蟾（FETAX）结果的大型数据库。（替代方法验证机构间协调委员会）倡议，我们发现，在某些情况下，报告的畸形率存在显着的实验室间变异性，似乎与研究者之间的诊断差异有关，基于这些结果，对于如何改进FETAX方案以减少这种可变性并促进该测定法的任何未来验证提出了具体建议。