SOLUTION SCATTERING FROM COMPACT, DENATURED FORMS OF STAPHYLOCOCCAL NUCLEASE

来自紧凑、变性形式的葡萄球菌核酸酶的溶液散射

• 批准号: 6437707
• 负责人: EATON E LATTMAN
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437707
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

We propose to make use of existing small angle X-ray scattering (SAXS) facilities at SSRL to carry out studies of protein folding intermediates encountered in mutants of staphylococcal nuclease and in the a subunit of tryptophan synthase. The studies push the limits of available technology both in terms of the time scale of kinetic experiments and in quality of the signal desired in the equilibrium experiments, where we hope to do quantitative analysis on samples containing mixtures of species. In particular we hope to use the new broad band pass multilayer recently tested on beamline 4-2 to increase intensity and decrease exposure times.

我们建议利用现有的小角X射线散射 （SAXS）设施进行蛋白质折叠的研究 在葡萄球菌核酸酶突变体中遇到的中间体， 色氨酸合成酶的a亚基。 这些研究突破了 现有的技术，无论是在时间尺度的动力学 实验和平衡中所需信号的质量 实验，我们希望对样品进行定量分析， 含有多种物种的混合物。 特别是我们希望使用新的 最近在光束线4-2上测试的宽带通多层膜， 强度和减少曝光时间。