SOLUTION SCATTERING FROM COMPACT, DENATURED FORMS OF STAPHYLOCOCCAL NUCLEASE

我们建议利用现有的小角X射线散射 (SAXS)在SSRL的设施进行蛋白质折叠研究 葡萄球菌核酸酶突变体中的中间体和 色氨酸合成酶的a亚基。这些研究突破了 现有技术在以下两个方面的时间尺度 实验和在均衡中所需的信号质量 实验，我们希望对样品进行定量分析 含有各种物种的混合物。特别是，我们希望使用新的 最近在光束线4-2上测试的宽带通过多层膜将增加 强度和减少曝光时间。

We propose to make use of existing small angle X-ray scattering (SAXS) facilities at SSRL to carry out studies of protein folding intermediates encountered in mutants of staphylococcal nuclease and in the a subunit of tryptophan synthase. The studies push the limits of available technology both in terms of the time scale of kinetic experiments and in quality of the signal desired in the equilibrium experiments, where we hope to do quantitative analysis on samples containing mixtures of species. In particular we hope to use the new broad band pass multilayer recently tested on beamline 4-2 to increase intensity and decrease exposure times.