SOLUTION SCATTERING FROM COMPACT, DENATURED FORMS OF STAPHYLOCOCCAL NUCLEASE

我们建议利用现有的小角度 X 射线散射 SSRL 的 (SAXS) 设施开展蛋白质折叠研究 葡萄球菌核酸酶突变体中遇到的中间体 色氨酸合酶的α亚基。 这些研究突破了极限 现有技术无论是在动力学的时间尺度上 实验和平衡所需信号的质量 实验，我们希望对样品进行定量分析 含有物种的混合物。 我们特别希望使用新的 最近在光束线 4-2 上测试了宽带通多层膜以增加 强度并减少曝光时间。

We propose to make use of existing small angle X-ray scattering (SAXS) facilities at SSRL to carry out studies of protein folding intermediates encountered in mutants of staphylococcal nuclease and in the a subunit of tryptophan synthase. The studies push the limits of available technology both in terms of the time scale of kinetic experiments and in quality of the signal desired in the equilibrium experiments, where we hope to do quantitative analysis on samples containing mixtures of species. In particular we hope to use the new broad band pass multilayer recently tested on beamline 4-2 to increase intensity and decrease exposure times.