High Speed Cell Sorter

高速细胞分选仪

• 批准号: 6440863
• 负责人: JAMES P ALLISON
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2003-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440863
• 关键词: biomedical equipment purchase; cell sorting; flow cytometry; high throughput technology

DESCRIPTION: Funds are requested to cover part of the purchase cost of a Cytomation MoFlo High Speed Cell Sorter. The instrument will be installed in the Flow Cytometry Facility of the Cancer Research Laboratory (CRL). The CRL will cover the remainder of the cost of the instrument and the cost of installation from non-federal sources and will provide administrative support for the instrument. The salary of a Staff Research Associated to operate the instrument will be covered by State funds administered by the CRL. Operating costs of the instrument will be borne by pro rata recharges to users. The CRL facility is the only flow cytometry facility on the U.C. Berkeley campus, and currently has a Coulter Elite sorter and three Coulter XL analytical sorters. The Facility is currently used by 42 laboratories, 25 of which use only the analyzers. Of the 17 laboratories that use the sorter, 5 major users account for 80 percent of the use. In the past year demand for sorter time has begun to regularly exceed capacity of our Elite sorter. The high-speed sorter will allow us to meet demand for sorting time. In addition, the instrument will allow us to use new techniques that are not possible on our current instrument, including 4 way sorting, analysis and sorting on more parameters, and experiments involving fluorescence resonance energy transfer.

说明：请拨资金用于支付购买一辆 Cytomation MoFlo高速细胞分选仪。仪器将安装在 癌症研究实验室（CRL）的流式细胞仪。的CRL 将支付仪器的剩余费用和 安装从非联邦来源，并将提供行政支持 对于仪器。一名研究助理人员的薪金， 文书的费用将由CRL管理的国家基金支付。操作 该仪器的费用将通过按比例向用户重新收费来承担。的CRL 是加州唯一的流式细胞仪设备伯克利分校，以及 目前拥有一台Coulter Elite分选机和三台Coulter XL分析分选机。 该设施目前有42个实验室使用，其中25个实验室仅使用 分析仪。在使用该分拣机的17个实验室中， 80%的使用。在过去的一年里，分拣机的时间需求开始增加， 经常超出我们Elite分拣机的容量。高速分拣机将允许 以满足分拣时间的需求。此外，该仪器将允许我们 使用我们现有仪器无法实现的新技术， 包括4路排序，更多参数的分析排序， 荧光共振能量转移实验。