CORE--CLINICAL AND MOUSE CORE

这个核心的目的是为PPG研究人员提供人类和小鼠的组织和核酸，以支持个别项目。具体地说，该核心将协调由艾滋病肾脏并发症联合会(RCAC)提供的临床样本的使用，RCAC是一个对HIVAN的发病机制感兴趣的合作肾病学家小组。RCAC已同意合作参与对接受肾活检的HIV-1血清阳性患者的研究。临床数据库和生物样本的集中储存库将促进本计划项目中提出的所有研究。从每个患者获得的血液和肾脏材料将被处理并储存或分发给适当的调查人员。核心将提供每个活组织标本的初步组织病理学分析，以用于拟议的研究。该核心将负责扩展和鉴定从这些患者的外周血液和肾脏活检中获得的初级病毒分离株。从研究患者和对照患者的外周血单核细胞中获得的B细胞将被EB病毒转化，以便为项目1中的遗传学研究提供持续可再生的DNA来源。项目2中将从不同遗传背景的患者的供体和身体标本中制备一组原代人肾小管细胞，用于项目2的体外研究。核心将为项目1和项目3提供转基因小鼠。这包括继续维护和杂交已建立的HIV-1转基因系(TG26)，以及创建携带项目1中所述单个HIV-1基因的新品系。该核心将提供肾功能监测以及这些线路的肾脏组织病理学。因此，核心B在为调查人员提供拟议项目所需的人和鼠试剂方面发挥了关键作用。

The purpose of this core is to provide PPG investigators with human and murine tissue and nucleic acid in support of the individual projects. Specifically, the core will coordinate the use of clinical samples provided by the Renal Complications of AIDS Consortium (RCAC), a group of collaborating nephrologists interested in the pathogenesis of HIVAN. The RCAC, has agreed to participate collaboratively in studies of HIV-1 seropositive patients undergoing renal biopsy. The clinical database and centralized repository of biological samples will facilitate all studies proposed in this program project. Blood and kidney material obtained from each patient will be processed and either stored or distributed to the appropriate investigators. The core will provide the initial histopathologic analysis of each biopsy specimen to be used in the proposed studies. The core will be responsible for expanding and characterizing primary viral isolates obtained from the peripheral blood and kidney biopsies of these patients. B-cells obtained from the peripheral blood mononuclear cells of study patients as well as controls will be transformed with Epstein-Barr virus to allow for a continuous renewable source of DNA for the genetic studies in project 1. A panel of primary human renal tubule cells will be prepared from donor and autopsy specimens of patients of differing genetic backgrounds to be used for the in vitro studies in project 2. The core will provide transgenic mice for projects 1 and 3. This includes the continual maintenance of and cross breeding of the established HIV-1 transgenic line (Tg26) as well as the creation of novel lines carrying single HIV-1 genes as described in project. The core will provide both renal function monitoring as well as renal histopathology of these lines. Thus Core B serves a critical function in providing investigators with the necessary human and mouse reagents for the proposed projects.