CORE--CLINICAL AND MOUSE CORE

该核心的目的是为PPG研究人员提供人类和小鼠组织和核酸，以支持各个项目。具体来说，该中心将协调使用艾滋病肾脏并发症联盟（RCAC）提供的临床样本，RCAC是一组对hiv发病机制感兴趣的合作肾病学家。RCAC已同意合作参与HIV-1血清阳性患者接受肾活检的研究。临床数据库和集中的生物样本库将促进本项目提出的所有研究。从每位患者获得的血液和肾脏材料将被处理并储存或分发给适当的研究人员。核心将提供用于拟议研究的每个活检标本的初始组织病理学分析。核心将负责扩大和鉴定从这些患者的外周血和肾脏活检中获得的原代病毒分离株。从研究患者和对照组的外周血单个核细胞中获得的b细胞将用爱泼斯坦-巴尔病毒转化，以便为项目1中的遗传研究提供连续的可再生DNA来源。将从不同遗传背景的患者的供体和尸检标本中制备一组原代人肾小管细胞，用于项目2的体外研究。该中心将为项目1和项目3提供转基因小鼠。这包括对已建立的HIV-1转基因品系（Tg26）的持续维护和杂交育种，以及项目中描述的携带单个HIV-1基因的新品系的创建。核心将提供肾功能监测以及这些系的肾脏组织病理学。因此，Core B在为研究人员提供拟议项目所需的人和小鼠试剂方面发挥着关键作用。

The purpose of this core is to provide PPG investigators with human and murine tissue and nucleic acid in support of the individual projects. Specifically, the core will coordinate the use of clinical samples provided by the Renal Complications of AIDS Consortium (RCAC), a group of collaborating nephrologists interested in the pathogenesis of HIVAN. The RCAC, has agreed to participate collaboratively in studies of HIV-1 seropositive patients undergoing renal biopsy. The clinical database and centralized repository of biological samples will facilitate all studies proposed in this program project. Blood and kidney material obtained from each patient will be processed and either stored or distributed to the appropriate investigators. The core will provide the initial histopathologic analysis of each biopsy specimen to be used in the proposed studies. The core will be responsible for expanding and characterizing primary viral isolates obtained from the peripheral blood and kidney biopsies of these patients. B-cells obtained from the peripheral blood mononuclear cells of study patients as well as controls will be transformed with Epstein-Barr virus to allow for a continuous renewable source of DNA for the genetic studies in project 1. A panel of primary human renal tubule cells will be prepared from donor and autopsy specimens of patients of differing genetic backgrounds to be used for the in vitro studies in project 2. The core will provide transgenic mice for projects 1 and 3. This includes the continual maintenance of and cross breeding of the established HIV-1 transgenic line (Tg26) as well as the creation of novel lines carrying single HIV-1 genes as described in project. The core will provide both renal function monitoring as well as renal histopathology of these lines. Thus Core B serves a critical function in providing investigators with the necessary human and mouse reagents for the proposed projects.