CORE--CLINICAL AND MOUSE CORE

该核心的目的是为PPG研究者提供人类和小鼠组织和核酸，以支持各个项目。具体来说，该核心将协调使用由艾滋病肾脏并发症联盟（RCAC）提供的临床样本，该联盟是一组对HIVAN发病机制感兴趣的合作肾脏病学家。 驻地协调员评估中心已同意合作参与对接受肾活检的HIV-1血清阳性患者的研究。 临床数据库和生物样本的集中储存库将促进本项目中提出的所有研究。 将对从每例患者中获得的血液和肾脏材料进行处理，并储存或分发给适当的研究者。 核心将提供拟用于拟定研究的每份活检标本的初始组织病理学分析。 核心将负责扩大和表征从这些患者的外周血和肾活检中获得的主要病毒分离株。 从研究患者和对照的外周血单核细胞中获得的B细胞将用EB病毒转化，以便为项目1中的遗传研究提供连续可再生的DNA来源。 将从不同遗传背景患者的供体和尸检标本中制备一组原代人肾小管细胞，用于项目2的体外研究。 核心将为项目1和3提供转基因小鼠。 这包括对已建立的HIV-1转基因系（Tg 26）进行持续维护和杂交育种，以及创建携带单个HIV-1基因的新系，如项目所述。 核心将提供这些管路的肾功能监测以及肾组织病理学。因此，核心B在为研究者提供拟定项目所需的人类和小鼠试剂方面发挥着关键作用。

The purpose of this core is to provide PPG investigators with human and murine tissue and nucleic acid in support of the individual projects. Specifically, the core will coordinate the use of clinical samples provided by the Renal Complications of AIDS Consortium (RCAC), a group of collaborating nephrologists interested in the pathogenesis of HIVAN. The RCAC, has agreed to participate collaboratively in studies of HIV-1 seropositive patients undergoing renal biopsy. The clinical database and centralized repository of biological samples will facilitate all studies proposed in this program project. Blood and kidney material obtained from each patient will be processed and either stored or distributed to the appropriate investigators. The core will provide the initial histopathologic analysis of each biopsy specimen to be used in the proposed studies. The core will be responsible for expanding and characterizing primary viral isolates obtained from the peripheral blood and kidney biopsies of these patients. B-cells obtained from the peripheral blood mononuclear cells of study patients as well as controls will be transformed with Epstein-Barr virus to allow for a continuous renewable source of DNA for the genetic studies in project 1. A panel of primary human renal tubule cells will be prepared from donor and autopsy specimens of patients of differing genetic backgrounds to be used for the in vitro studies in project 2. The core will provide transgenic mice for projects 1 and 3. This includes the continual maintenance of and cross breeding of the established HIV-1 transgenic line (Tg26) as well as the creation of novel lines carrying single HIV-1 genes as described in project. The core will provide both renal function monitoring as well as renal histopathology of these lines. Thus Core B serves a critical function in providing investigators with the necessary human and mouse reagents for the proposed projects.