CORE--CLINICAL AND MOUSE CORE

核心——临床和鼠标核心

• 批准号: 6655210
• 负责人: Mary E. Klotman
• 金额: $ 22.85万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6655210
• 关键词: HIV infections; animal breeding; animal colony; animal tissue; biomedical facility; biopsy; chronic renal failure; clinical research; gene targeting; genetically modified animals; histopathology; human genetic material tag; human tissue; laboratory mouse; medical complication; patient /disease registry; tissue resource /registry

The purpose of this core is to provide PPG investigators with human and murine tissue and nucleic acid in support of the individual projects. Specifically, the core will coordinate the use of clinical samples provided by the Renal Complications of AIDS Consortium (RCAC), a group of collaborating nephrologists interested in the pathogenesis of HIVAN. The RCAC, has agreed to participate collaboratively in studies of HIV-1 seropositive patients undergoing renal biopsy. The clinical database and centralized repository of biological samples will facilitate all studies proposed in this program project. Blood and kidney material obtained from each patient will be processed and either stored or distributed to the appropriate investigators. The core will provide the initial histopathologic analysis of each biopsy specimen to be used in the proposed studies. The core will be responsible for expanding and characterizing primary viral isolates obtained from the peripheral blood and kidney biopsies of these patients. B-cells obtained from the peripheral blood mononuclear cells of study patients as well as controls will be transformed with Epstein-Barr virus to allow for a continuous renewable source of DNA for the genetic studies in project 1. A panel of primary human renal tubule cells will be prepared from donor and autopsy specimens of patients of differing genetic backgrounds to be used for the in vitro studies in project 2. The core will provide transgenic mice for projects 1 and 3. This includes the continual maintenance of and cross breeding of the established HIV-1 transgenic line (Tg26) as well as the creation of novel lines carrying single HIV-1 genes as described in project. The core will provide both renal function monitoring as well as renal histopathology of these lines. Thus Core B serves a critical function in providing investigators with the necessary human and mouse reagents for the proposed projects.

该核心的目的是为PPG研究人员提供人类和鼠组织和核酸，以支持各个项目。具体而言，核心将协调艾滋病联盟（RCAC）肾脏并发症提供的临床样本，这是一群对Hivan发病机理感兴趣的合作肾脏病师。 RCAC已同意合作参加接受肾脏活检的HIV-1血清阳性患者。 生物样品的临床数据库和集中式存储库将有助于该计划项目中提出的所有研究。 将处理从每个患者获得的血液和肾脏材料，并存储或分配给适当的研究人员。 核心将提供每个活检标本的初步组织病理学分析，该试样将用于拟议的研究。 核心将负责扩大和表征从这些患者的外周血和肾脏活检中获得的原发性病毒株。 从研究患者的周围血液单核细胞获得的B细胞以及对照组将与爱泼斯坦 - 巴尔病毒转化，以允许项目1中的遗传研究的连续可再生DNA来源。原代人肾小管细胞的遗传研究。项目1和3。这包括既定的HIV-1转基因线（TG26）的持续维护和交叉育种，以及如项目所述的新型携带单个HIV-1基因的新型线条的创建。 核心将提供肾功能监测以及这些线的肾脏组织病理学。因此，核心B在为研究人员提供所提出的项目的必要人力和小鼠试剂时起着关键功能。