DIETARY CONTENT & GASTROINTESTINAL ABSORPTION OF D CHIRO INOSITOL & PINITOL

膳食含量

• 批准号: 6486757
• 负责人: RICHARD E. OSTLUND
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486757
• 关键词: biological products; biomedical resource; human tissue; immunology; inflammation; proteins; spectrometry

D-chiro-Inositol-containing compounds were abundant in common laboratory animal feed. By gas chromatography-mass spectrometry (GC/MS) of 6 N HCI hydrolysates, Purina Roden Chow 5001 contained 0.23% of D-chiro-inositol by weight. The principal sources were alfalfa and soybean meal whereas other chow ingredients had less than 2% as much per gram as these legumes. Only traces of L-chiro-inositol were found. D-chiro-Inositol was present in a water-soluble non-ionic form of which most was shown to be pinitol, 1D-3-O-methyl-chiro-inositol. More than 98% of the D-chiro-Inositol fed to rats as Purina Rodent Chow 5001 as well as purified pinitol and deuterated D-chiro-Inositol tracer was absorbed from the gastrointestinal tract. Rats chronically ingesting the chow diet consumed 921 (mol of total D-chiro-inositol/kg/day and excreted less than 5% of that amount in the stool and urine suggesting that the bulk was metabolized. However, even though urinary excretion of total D-chiro-i nositol was very small compared to intake, it declined further by over 98% in response to a chiro-inositol-deficient diet. Thus, urinary levels may reflect dietary intake. Recent reports have shown that D-chiro-inositol is found in certain putative insulin mediator fractions and that increased urinary excretion occurs in uncontroleld diabetes mellitus. Further work is needed to clarify the dietary and potential nutritive role of D-chiro-inositol.

含有D-二氢肌醇的化合物种类繁多 实验室动物饲料。气相色谱-质谱法 (GC/MS)6 N-盐酸水解物，Purina Roden Chow 5001含有 0.23%的D-甲氧基肌醇。其主要来源是 紫花苜蓿和豆粕，而其他食物成分的含量低于 每克是这些豆类的2%。只有L次郎肌醇的痕迹 都被发现了。D-氯代肌醇存在于一种水溶性非离子化合物中 其中大部分被证明是皮尼托， 1D-3-O-甲基肌醇。超过98%的D-环肌醇 给大鼠喂食紫苏啮齿动物Chow 5001以及纯化的松油醇和 氢化D-二氢-肌醇示踪剂是从 胃肠道。长期摄入食物的大鼠 每天摄入921(摩尔)总D-肌醇/公斤和排泄量较少 在粪便和尿液中的含量超过5%，这表明 被新陈代谢。然而，即使尿液总排泄量 与摄入量相比，D-chiro-i nositol非常小，它下降了 此外，对缺乏肌醇的饮食的反应超过98%。 因此，尿液水平可能反映饮食摄入量。最近的报道说 研究表明，在某些假定的胰岛素中发现了D-环状肌醇 介质组分和尿液排泄量增加发生在 无法控制的糖尿病。还需要进一步的工作来澄清 D-甲氧基肌醇的膳食作用和潜在营养作用。