cDNA Microarray Reader Instrumentation

cDNA 微阵列阅读器仪器

• 批准号: 6548620
• 负责人: Paul D Smith
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6548620
• 关键词: biomedical equipment development; complementary DNA; confocal scanning microscopy; fluorescence; gene expression; microarray technology; nucleic acid hybridization

cDNA micro-array readers are invaluable tools for analysis of gene expression and for gene discovery through analysis of the fluorescent patterns of probes hybridized to a library of cDNA targets immobilized on a microscope slide. The fluorescence diagnostic instrumentation is based on a scanning confocal microscope in which laser excited fluorescence is spatially filtered and detected using a photomultiplier. Evaluation of 16,000 different gene fragments is possible on a single microscope slide. Identification of unwanted, background fluorescence signals with modifications to the optical filtration has resulted in a significant improvement to the signal to noise ratio.Thus, lower levels of gene expression can now be determined.

cDNA微阵列阅读器是用于分析基因表达和通过分析与固定在显微镜载玻片上的cDNA靶文库杂交的探针的荧光图案进行基因发现的宝贵工具。荧光诊断仪器基于扫描共聚焦显微镜，其中激光激发的荧光被空间过滤并使用光电倍增管检测。在一张显微镜载玻片上可以评估16，000个不同的基因片段。通过对光学过滤的修改，识别不需要的背景荧光信号，导致信噪比的显著改善，因此，现在可以确定较低水平的基因表达。