GENE TARGETING APPROACHES TO MCH AND ENERGY BALANCE

描述：（改编自申请人摘要）本研究拟 描述MCH基因敲除小鼠的瘦型，并确定 MCH和衍生自MCH的另外两种肽的相对贡献 基因、N-EI和N-GE。为了确定MCH在介导食欲产生中的作用， 或其他神经肽的食欲抑制作用 将评估MCH-/-中的调节肽。除了检查 在啮齿动物肥胖的单基因模型中介导肥胖的重要性， MCH-/-小鼠将与瘦素缺陷（ob/ob）小鼠和瘦素缺乏（ob/ob）小鼠杂交。 耐药A（agglutinase）小鼠。MCH-/-小鼠将与消融小鼠杂交 NPY或食欲素，其他参与能量平衡的神经肽。最后到 确定MCH在介导肥胖中的重要性， 环境因素，高脂肪饮食，味精和GTG引起 将在野生型和MCH-/-动物中比较肥胖。

DESCRIPTION: (Adapted from the applicant's abstract) This study proposes to characterize the lean phenotype in MCH knockout mice, and to ascertain the relative contributions of MCH and the two other peptides derived from the MCH gene, N-EI and N-GE. To determine the role of MCH in mediating the orexigenic or appetite inhibiting action of other neuropeptides the effect of appetite regulating peptides in MCH-/-will be assessed. In addition to examining the importance of mediating obesity in single gene models of rodent obesity, MCH-/-mice will be crossbred to leptin deficient (ob/ob) mice and leptin resistant A (agouti) mice. MCH-/- mice will be crossbred to mice with ablations NPY or orexin, other neuropeptides implicated in energy balance. Finally to determine the importance of MCH in mediating obesity in response to environmental agents, the ability of a high fat diet, MSG and GTG to cause obesity will be compared in wild type and MCH-/- animals.